Standardization of modified allergens using polymerized ragweed as a model system

Leslie C. Grammer*, Martha A. Shaughnessy, Roy Patterson

*Corresponding author for this work

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Polymerized allergens are manufactured by crosslinking allergen molecules with glutaraldehyde and then selecting a desired molecular weight range by gel filtration chromatography. Polymerized allergen extracts have retained antigenicity but reduced allergenicity when compared with conventional allergenic extracts. Thus, the important parameters to standardize in polymerized extracts are allergenicity, antigenicity, and molecular weight range. As our initial studies have demonstrated that allergenicity correlates with antigenicity and molecular weight, only the latter are necessary to measure. In short, polymerized allergen extracts could be standardized simply in terms of in vitro reactivity and chromatographic profile.

Original languageEnglish (US)
Pages (from-to)107-116
Number of pages10
JournalClinical Reviews In Allergy
Volume5
Issue number1
DOIs
StatePublished - Feb 1 1987

ASJC Scopus subject areas

  • Immunology and Allergy

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