Standardized methods and quality control limits for agar and broth microdilution susceptibility testing of Mycoplasma pneumoniae, Mycoplasma hominis, and Ureaplasma urealyticum

Ken B. Waites*, Lynn B. Duffy, Cécile M. Bébéar, Anne Matlow, Deborah F. Talkington, George E. Kenny, Patricia A. Totten, Donald J. Bade, Xiaotian Zheng, Maureen K. Davidson, Virginia D. Shortridge, Jeffrey L. Watts, Steven D. Brown

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

58 Scopus citations

Abstract

An international multilaboratory collaborative study was conducted to develop standard media and consensus methods for the performance and quality control of antimicrobial susceptibility testing of Mycoplasma pneumoniae, Mycoplasma hominis, and Ureaplasma urealyticum using broth microdilution and agar dilution techniques. A reference strain from the American Type Culture Collection was designated for each species, which was to be used for quality control purposes. Repeat testing of replicate samples of each reference strain by participating laboratories utilizing both methods and different lots of media enabled a 3- to 4-dilution MIC range to be established for drugs in several different classes, including tetracyclines, macrolides, ketolides, lincosamides, and fluoroquinolones. This represents the first multilaboratory collaboration to standardize susceptibility testing methods and to designate quality control parameters to ensure accurate and reliable assay results for mycoplasmas and ureaplasmas that infect humans.

Original languageEnglish (US)
Pages (from-to)3542-3547
Number of pages6
JournalJournal of clinical microbiology
Volume50
Issue number11
DOIs
StatePublished - Nov 2012

ASJC Scopus subject areas

  • Microbiology (medical)

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