STAT6-independent production of IL-4 by mast cells

Melanie A. Sherman, Virginia H. Secor, Susan K. Lee, Richard D. Lopez, Melissa A. Brown*

*Corresponding author for this work

Research output: Contribution to journalArticle

40 Scopus citations

Abstract

The acquisition of an IL-4-producing phenotype in Th2 cells requires IL-4 signaling through the STAT6 pathway during T cell differentiation. In this study we demonstrate that, unlike in naive T cells, IL-4 is not necessary for the development of an IL-4-producing phenotype in mast cells. Bone marrow-derived mast cell precursors from STATG(-/-) mice can differentiate into mature cells that express IL-4 levels comparable to those of wild-type mast cells. In differentiated mast cells, activation in the presence of neutralizing anti-IL-4 antibodies or mutation of the consensus STAT6 sites does not diminish IL-4 promoter activity, indicating that IL-4 is not required for active transcription. Taken together these data suggest that mast cell IL-4 production is not STAT6 dependent, providing evidence that these cells could generate IL-4 needed for the initiation and amplification of an effective Th2 immune response.

Original languageEnglish (US)
Pages (from-to)1235-1242
Number of pages8
JournalEuropean Journal of Immunology
Volume29
Issue number4
DOIs
StatePublished - 1999

Keywords

  • Cytokine
  • Mast cell
  • Signal transduction
  • Transcription factor

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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