Stem cell factor influences neuro-immune interactions: The response of mast cells to pituitary adenylate cyclase activating polypeptide is altered by stem cell factor

A. Schmidt-Choudhury; J. Meissner; J. Seebeck; E. J. Goetzl; M. Xia; S. J. Galli; W. E. Schmidt; J. Schaub; B. K. Wershil

doi:10.1016/S0167-0115(99)00054-3

Stem cell factor influences neuro-immune interactions: The response of mast cells to pituitary adenylate cyclase activating polypeptide is altered by stem cell factor

A. Schmidt-Choudhury^*, J. Meissner, J. Seebeck, E. J. Goetzl, M. Xia, S. J. Galli, W. E. Schmidt, J. Schaub, B. K. Wershil

^*Corresponding author for this work

Pediatrics

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

Mast cells degranulation can be elicited by a number of biologically important neuropeptides, but the mechanisms involved in mast cell-neuropeptide interactions have not been fully elucidated. Stem cell factor (SCF), also known as c-kit or kit ligand, induces multiple effects on mast cells, including proliferation, differentiation, maturation, and prevents apoptosis. We investigated the ability of SCF to affect mast cell responsiveness to the neuropeptides pituitary adenylate cyclase activating polypeptide (PACAP) and vasoactive intestinal peptide (VIP). PACAP 1-27, PACAP1-38, or VIP failed to induced preformed mediator release from mouse bone-marrow-cultured mast cells (BMCMC) derived in concanavalin A-stimulated spleen conditioned medium (CM). By contrast, BMCMC grown in SCF-containing medium or freshly isolated peritoneal mast cells exhibited significant ³H-hydroxytrypamine (5-HT) release in response to PACAP peptides or VIP. Deoxyglucose and the mitochondrial inhibitor antimycin significantly inhibited PACAP-induced 5-HT release indicating that the central event induced by PACAP peptides was exocytosis. The G(α)i inhibitor, pertussis toxin, significantly diminished PACAP-induced 5-HT release from BMCMCs in SCF suggesting the involvement of heterotrimeric G-proteins. Western blot analysis using antibodies directed against the human VIP type I/PACAP type II receptor demonstrated a 70-72 kD immunoreactive protein expressed in greater amounts in BMCMC grown in SCF compared with BMCMC in CM. We conclude that SCF induces a mast cell population that is responsive to PACAPs and VIP involving a heterotrimeric G-protein-dependent mechanism. Copyright (C) 1999 Elsevier Science B.V.

Original language	English (US)
Pages (from-to)	73-80
Number of pages	8
Journal	Regulatory Peptides
Volume	83
Issue number	2-3
DOIs	https://doi.org/10.1016/S0167-0115(99)00054-3
State	Published - 1999

Funding

We thank Ingrid Berg and Jackie A. Lavigne for expert technical assistance. Rat recombinant stem cell factor was kindly provided by Dr. K. Langley (AMGEN, Thousand Oaks, CA). This work was supported by NIH grants DK33506 and DK46819 (B.K.W.), AI 29912 (E.J.G.) and CA72074 and AI23990 (S.J.G.) and grants from the Deutsche Forschungsgemeinschaft Schm 805/4-2 (W.E.S.) Ro 819-1/1 and Schm 1223/4-1(A.S.-C.)

Keywords

Mast cells
Neuropeptides
Pituitary adenylate cyclase activating polypeptide
Vasoactive intestinal polypeptide

ASJC Scopus subject areas

Endocrinology
Cellular and Molecular Neuroscience
Biochemistry
Physiology
Clinical Biochemistry

Access to Document

10.1016/S0167-0115(99)00054-3

Cite this

Schmidt-Choudhury, A., Meissner, J., Seebeck, J., Goetzl, E. J., Xia, M., Galli, S. J., Schmidt, W. E., Schaub, J., & Wershil, B. K. (1999). Stem cell factor influences neuro-immune interactions: The response of mast cells to pituitary adenylate cyclase activating polypeptide is altered by stem cell factor. Regulatory Peptides, 83(2-3), 73-80. https://doi.org/10.1016/S0167-0115(99)00054-3

@article{480c6b2fb4284b4b98ea896db3478f62,

title = "Stem cell factor influences neuro-immune interactions: The response of mast cells to pituitary adenylate cyclase activating polypeptide is altered by stem cell factor",

abstract = "Mast cells degranulation can be elicited by a number of biologically important neuropeptides, but the mechanisms involved in mast cell-neuropeptide interactions have not been fully elucidated. Stem cell factor (SCF), also known as c-kit or kit ligand, induces multiple effects on mast cells, including proliferation, differentiation, maturation, and prevents apoptosis. We investigated the ability of SCF to affect mast cell responsiveness to the neuropeptides pituitary adenylate cyclase activating polypeptide (PACAP) and vasoactive intestinal peptide (VIP). PACAP 1-27, PACAP1-38, or VIP failed to induced preformed mediator release from mouse bone-marrow-cultured mast cells (BMCMC) derived in concanavalin A-stimulated spleen conditioned medium (CM). By contrast, BMCMC grown in SCF-containing medium or freshly isolated peritoneal mast cells exhibited significant 3H-hydroxytrypamine (5-HT) release in response to PACAP peptides or VIP. Deoxyglucose and the mitochondrial inhibitor antimycin significantly inhibited PACAP-induced 5-HT release indicating that the central event induced by PACAP peptides was exocytosis. The G(α)i inhibitor, pertussis toxin, significantly diminished PACAP-induced 5-HT release from BMCMCs in SCF suggesting the involvement of heterotrimeric G-proteins. Western blot analysis using antibodies directed against the human VIP type I/PACAP type II receptor demonstrated a 70-72 kD immunoreactive protein expressed in greater amounts in BMCMC grown in SCF compared with BMCMC in CM. We conclude that SCF induces a mast cell population that is responsive to PACAPs and VIP involving a heterotrimeric G-protein-dependent mechanism. Copyright (C) 1999 Elsevier Science B.V.",

keywords = "Mast cells, Neuropeptides, Pituitary adenylate cyclase activating polypeptide, Vasoactive intestinal polypeptide",

author = "A. Schmidt-Choudhury and J. Meissner and J. Seebeck and Goetzl, {E. J.} and M. Xia and Galli, {S. J.} and Schmidt, {W. E.} and J. Schaub and Wershil, {B. K.}",

note = "Funding Information: We thank Ingrid Berg and Jackie A. Lavigne for expert technical assistance. Rat recombinant stem cell factor was kindly provided by Dr. K. Langley (AMGEN, Thousand Oaks, CA). This work was supported by NIH grants DK33506 and DK46819 (B.K.W.), AI 29912 (E.J.G.) and CA72074 and AI23990 (S.J.G.) and grants from the Deutsche Forschungsgemeinschaft Schm 805/4-2 (W.E.S.) Ro 819-1/1 and Schm 1223/4-1(A.S.-C.) ",

year = "1999",

doi = "10.1016/S0167-0115(99)00054-3",

language = "English (US)",

volume = "83",

pages = "73--80",

journal = "Regulatory Peptides",

issn = "0167-0115",

publisher = "Elsevier",

number = "2-3",

}

Schmidt-Choudhury, A, Meissner, J, Seebeck, J, Goetzl, EJ, Xia, M, Galli, SJ, Schmidt, WE, Schaub, J & Wershil, BK 1999, 'Stem cell factor influences neuro-immune interactions: The response of mast cells to pituitary adenylate cyclase activating polypeptide is altered by stem cell factor', Regulatory Peptides, vol. 83, no. 2-3, pp. 73-80. https://doi.org/10.1016/S0167-0115(99)00054-3

TY - JOUR

T1 - Stem cell factor influences neuro-immune interactions

T2 - The response of mast cells to pituitary adenylate cyclase activating polypeptide is altered by stem cell factor

AU - Schmidt-Choudhury, A.

AU - Meissner, J.

AU - Seebeck, J.

AU - Goetzl, E. J.

AU - Xia, M.

AU - Galli, S. J.

AU - Schmidt, W. E.

AU - Schaub, J.

AU - Wershil, B. K.

N1 - Funding Information: We thank Ingrid Berg and Jackie A. Lavigne for expert technical assistance. Rat recombinant stem cell factor was kindly provided by Dr. K. Langley (AMGEN, Thousand Oaks, CA). This work was supported by NIH grants DK33506 and DK46819 (B.K.W.), AI 29912 (E.J.G.) and CA72074 and AI23990 (S.J.G.) and grants from the Deutsche Forschungsgemeinschaft Schm 805/4-2 (W.E.S.) Ro 819-1/1 and Schm 1223/4-1(A.S.-C.)

PY - 1999

Y1 - 1999

N2 - Mast cells degranulation can be elicited by a number of biologically important neuropeptides, but the mechanisms involved in mast cell-neuropeptide interactions have not been fully elucidated. Stem cell factor (SCF), also known as c-kit or kit ligand, induces multiple effects on mast cells, including proliferation, differentiation, maturation, and prevents apoptosis. We investigated the ability of SCF to affect mast cell responsiveness to the neuropeptides pituitary adenylate cyclase activating polypeptide (PACAP) and vasoactive intestinal peptide (VIP). PACAP 1-27, PACAP1-38, or VIP failed to induced preformed mediator release from mouse bone-marrow-cultured mast cells (BMCMC) derived in concanavalin A-stimulated spleen conditioned medium (CM). By contrast, BMCMC grown in SCF-containing medium or freshly isolated peritoneal mast cells exhibited significant 3H-hydroxytrypamine (5-HT) release in response to PACAP peptides or VIP. Deoxyglucose and the mitochondrial inhibitor antimycin significantly inhibited PACAP-induced 5-HT release indicating that the central event induced by PACAP peptides was exocytosis. The G(α)i inhibitor, pertussis toxin, significantly diminished PACAP-induced 5-HT release from BMCMCs in SCF suggesting the involvement of heterotrimeric G-proteins. Western blot analysis using antibodies directed against the human VIP type I/PACAP type II receptor demonstrated a 70-72 kD immunoreactive protein expressed in greater amounts in BMCMC grown in SCF compared with BMCMC in CM. We conclude that SCF induces a mast cell population that is responsive to PACAPs and VIP involving a heterotrimeric G-protein-dependent mechanism. Copyright (C) 1999 Elsevier Science B.V.

AB - Mast cells degranulation can be elicited by a number of biologically important neuropeptides, but the mechanisms involved in mast cell-neuropeptide interactions have not been fully elucidated. Stem cell factor (SCF), also known as c-kit or kit ligand, induces multiple effects on mast cells, including proliferation, differentiation, maturation, and prevents apoptosis. We investigated the ability of SCF to affect mast cell responsiveness to the neuropeptides pituitary adenylate cyclase activating polypeptide (PACAP) and vasoactive intestinal peptide (VIP). PACAP 1-27, PACAP1-38, or VIP failed to induced preformed mediator release from mouse bone-marrow-cultured mast cells (BMCMC) derived in concanavalin A-stimulated spleen conditioned medium (CM). By contrast, BMCMC grown in SCF-containing medium or freshly isolated peritoneal mast cells exhibited significant 3H-hydroxytrypamine (5-HT) release in response to PACAP peptides or VIP. Deoxyglucose and the mitochondrial inhibitor antimycin significantly inhibited PACAP-induced 5-HT release indicating that the central event induced by PACAP peptides was exocytosis. The G(α)i inhibitor, pertussis toxin, significantly diminished PACAP-induced 5-HT release from BMCMCs in SCF suggesting the involvement of heterotrimeric G-proteins. Western blot analysis using antibodies directed against the human VIP type I/PACAP type II receptor demonstrated a 70-72 kD immunoreactive protein expressed in greater amounts in BMCMC grown in SCF compared with BMCMC in CM. We conclude that SCF induces a mast cell population that is responsive to PACAPs and VIP involving a heterotrimeric G-protein-dependent mechanism. Copyright (C) 1999 Elsevier Science B.V.

KW - Mast cells

KW - Neuropeptides

KW - Pituitary adenylate cyclase activating polypeptide

KW - Vasoactive intestinal polypeptide

UR - http://www.scopus.com/inward/record.url?scp=0032783513&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032783513&partnerID=8YFLogxK

U2 - 10.1016/S0167-0115(99)00054-3

DO - 10.1016/S0167-0115(99)00054-3

M3 - Article

C2 - 10511460

AN - SCOPUS:0032783513

SN - 0167-0115

VL - 83

SP - 73

EP - 80

JO - Regulatory Peptides

JF - Regulatory Peptides

IS - 2-3

ER -

Stem cell factor influences neuro-immune interactions: The response of mast cells to pituitary adenylate cyclase activating polypeptide is altered by stem cell factor

Abstract

Funding

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this