TY - JOUR
T1 - Stem cells and platelet-rich plasma enhance the healing process of tendinitis in mice
AU - Fedato, Rosangela Alquieri
AU - Francisco, Júlio César
AU - Sliva, Gabriel
AU - De Noronha, Lúcia
AU - Olandoski, Márcia
AU - Neto, Jose Rocha Faria
AU - Ferreira, Priscila Elias
AU - Simeoni, Rossana Baggio
AU - Abdelwahid, Eltyeb
AU - De Carvalho, Katherine Athayde Teixeira
AU - Guarita-Souza, Luiz César
N1 - Publisher Copyright:
Copyright © 2019 Rosangela Alquieri Fedato et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
PY - 2019
Y1 - 2019
N2 - Objective. Achilles tendon pathologies occur frequently and have a significant socioeconomic impact. Currently, there is no evidence on the best treatment for these pathologies. Cell therapy has been studied in several animal models, and encouraging results have been observed with respect to tissue regeneration. This study is aimed at evaluating the functional and histological effects of bone marrow stem cell or platelet-rich plasma implantation compared to eccentric training in the treatment of Achilles tendinopathy in rats. Methods. Fourty-one male Wistar rats received collagenase injections into their bilateral Achilles tendons (collagenase-induced tendinopathy model). The rats were randomly divided into four groups: stem cells (SC), platelet-rich plasma (PRP), stem cells+platelet-rich plasma (SC+PRP), and control (eccentric training (ET)). After 4 weeks, the Achilles tendons were excised and subjected to biomechanical and histological analyses (Sirius red and hematoxylin-eosin staining). Results. Biomechanical assessments revealed no differences among the groups in ultimate tensile strength or yield strength of the tendons (p = 0 157), but there were significant differences in the elastic modulus (MPa; p = 0 044) and maximum tensile deformation (p = 0 005). The PRP group showed the greatest maximum deformation, and the SC group showed the highest Young’s modulus (elasticity) measurement. In histological analysis (hematoxylin-eosin and Sirius red staining), there were no differences among the groups. Conclusion. PRP and SC+PRP yielded better biomechanical results than eccentric training, showing that these treatments offer better tend function outcomes. This theoretical rationale for the belief that cell therapies can serve as viable alternatives to current treatments chronic fibrotic opens the door for opportunities to continue this research.
AB - Objective. Achilles tendon pathologies occur frequently and have a significant socioeconomic impact. Currently, there is no evidence on the best treatment for these pathologies. Cell therapy has been studied in several animal models, and encouraging results have been observed with respect to tissue regeneration. This study is aimed at evaluating the functional and histological effects of bone marrow stem cell or platelet-rich plasma implantation compared to eccentric training in the treatment of Achilles tendinopathy in rats. Methods. Fourty-one male Wistar rats received collagenase injections into their bilateral Achilles tendons (collagenase-induced tendinopathy model). The rats were randomly divided into four groups: stem cells (SC), platelet-rich plasma (PRP), stem cells+platelet-rich plasma (SC+PRP), and control (eccentric training (ET)). After 4 weeks, the Achilles tendons were excised and subjected to biomechanical and histological analyses (Sirius red and hematoxylin-eosin staining). Results. Biomechanical assessments revealed no differences among the groups in ultimate tensile strength or yield strength of the tendons (p = 0 157), but there were significant differences in the elastic modulus (MPa; p = 0 044) and maximum tensile deformation (p = 0 005). The PRP group showed the greatest maximum deformation, and the SC group showed the highest Young’s modulus (elasticity) measurement. In histological analysis (hematoxylin-eosin and Sirius red staining), there were no differences among the groups. Conclusion. PRP and SC+PRP yielded better biomechanical results than eccentric training, showing that these treatments offer better tend function outcomes. This theoretical rationale for the belief that cell therapies can serve as viable alternatives to current treatments chronic fibrotic opens the door for opportunities to continue this research.
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U2 - 10.1155/2019/1497898
DO - 10.1155/2019/1497898
M3 - Article
C2 - 31662764
AN - SCOPUS:85071287517
SN - 1687-966X
VL - 2019
JO - Stem Cells International
JF - Stem Cells International
M1 - 1497898
ER -