Stem cells from human fat as cellular delivery vehicles in an athymic rat posterolateral spine fusion model

Wellington K. Hsu, Jeffrey C. Wang, Nancy Q. Liu, Lucie Krenek, Patricia A. Zuk, Marc H. Hedrick, Prosper Benhaim, Jay R. Lieberman

Research output: Contribution to journalArticlepeer-review

88 Scopus citations


Background: Mesenchymal stem cells derived from human liposuction aspirates, termed processed lipoaspirate cells, have been utilized as cellular delivery vehicles for the induction of bone formation in tissue engineering and gene therapy strategies. In this study, we sought to evaluate the efficacy of bone morphogenetic protein (BMP)-2-producing adipose-derived stem cells in inducing a posterolateral spine fusion in an athymic rat model. Methods: Single-level (L4-L5) intertransverse spinal arthrodesis was attempted with use of a type-I collagen matrix in five groups of athymic rats, with eight animals in each group. Group I was treated with 5 × 106 adipose-derived stem cells transduced with an adenoviral vector containing the BMP-2 gene; group II, with 5 × 106 adipose-derived stem cells treated with osteogenic media and 1 μg/mL of recombinant BMP-2 (rhBMP-2); group III, with 10 μg of rhBMP-2; group IV, with 1 μg of rhBMP-2; and group V, with 5 × 106 adipose-derived stem cells alone. The animals that showed radiographic evidence of healing were killed four weeks after cell implantation and were examined with plain radiographs, manual palpation, microcomputed tomography scanning, and histological analysis. Results: All eight animals in group I demonstrated successful spinal fusion, with a large fusion mass, four weeks postoperatively. Furthermore, group-I specimens consistently revealed spinal fusion at the cephalad level (L3 and L4), where no fusion bed had been prepared surgically. In contrast, despite substantial BMP-2 production measured in vitro, group-II animals demonstrated minimal bone formation even eight weeks after implantation. Of the groups treated with the application of rhBMP-2 alone, the one that received a relatively high dose (group III) had a higher rate of fusion (seen in all eight specimens) than the one that received the low dose (group IV, in which fusion was seen in four of the eight specimens). None of the group-V animals (treated with adipose-derived stem cells alone) demonstrated successful spine fusion eight weeks after the surgery. Conclusions: Adipose-derived stem cells show promise as gene transduction targets for inducing bone formation to enhance spinal fusion in biologically stringent environments. Clinical Relevance: Adipose-derived stem cells demonstrate potential as cellular vehicles for the delivery of recombinant protein in a regional gene therapy strategy to enhance spinal fusion. However, before the exclusive use of adipose-derived stem cells in a cell-based strategy can become routine, more extensive study of the biological potential, the interactions between different carriers, and the influence of host biology is necessary.

Original languageEnglish (US)
Pages (from-to)1043-1052
Number of pages10
JournalThe Journal of bone and joint surgery. American volume
Issue number5
StatePublished - May 2008

ASJC Scopus subject areas

  • Surgery
  • Orthopedics and Sports Medicine


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