Stimulated Raman photoacoustic imaging

Vladislav V. Yakovlev, Hao F. Zhang, Gary D. Noojin, Michael L. Denton, Robert J. Thomas, Marlan O. Scully

Research output: Contribution to journalArticlepeer-review

74 Scopus citations

Abstract

Achieving label-free, molecular-specific imaging with high spatial resolution in deep tissue is often considered the grand challenge of optical imaging. To accomplish this goal, significant optical scattering in tissues has to be overcome while achieving molecular specificity without resorting to extrinsic labeling. We demonstrate the feasibility of developing such an optical imaging modality by combining the molecularly specific stimulated Raman excitation with the photoacoustic detection. By employing two ultrashort excitation laser pulses, separated in frequency by the vibrational frequency of a targeted molecule, only the specific vibrational level of the target molecules in the illuminated tissue volume is excited. This targeted optical absorption generates ultrasonic waves (referred to as stimulated Raman photoacoustic waves) which are detected using a traditional ultrasonic transducer to form an image following the design of the established photoacoustic microscopy.

Original languageEnglish (US)
Pages (from-to)20335-20339
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume107
Issue number47
DOIs
StatePublished - Nov 23 2010

Keywords

  • Biomedical imaging
  • Microscopy
  • Nonlinear optics
  • Photoacoustics

ASJC Scopus subject areas

  • General

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