Stimulation of colonic secretion by lipoxygenase metabolites of arachidonic acid

Mark W. Musch; Richard J. Miller; Michael Field; Marvin I. Siegel

doi:10.1126/science.6810465

Stimulation of colonic secretion by lipoxygenase metabolites of arachidonic acid

Mark W. Musch^*, Richard J. Miller, Michael Field, Marvin I. Siegel

^*Corresponding author for this work

Pharmacology

Research output: Contribution to journal › Article › peer-review

72 Scopus citations

Abstract

Both 5-hydroperoxyeicosatetraenoic acid (5-HPETE) and 5- hydroxyeicosatetraenoic acid (5-HETE) increased the short-circuit current (I_sc) in rabbit colonic mucosa mounted in vitro in Ussing chambers. Measurements of chlorine-36 fluxes indicated that the I_sc response to 5-HPETE is due to stimulation of active chlorine secretion. 9-, 11-, and 12-HPETE's and leukotrienes C₄ and B₄ produced either very small increases in I_sc or no increase. In contrast to results in rabbit colon, no HPETE, HETE, or leukotriene was effective in rabbit ileal mucosa. The effects of 5-HPETE in the rabbit colon were unaffected by mepacrine, but could be partially blocked by indomethacin. These results suggest that drugs which block both cyclooxygenase and lipoxygenase may be effective antidiarrheals in patients with colitis.

Original language	English (US)
Pages (from-to)	1255-1256
Number of pages	2
Journal	Science
Volume	217
Issue number	4566
DOIs	https://doi.org/10.1126/science.6810465
State	Published - 1982

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title = "Stimulation of colonic secretion by lipoxygenase metabolites of arachidonic acid",

abstract = "Both 5-hydroperoxyeicosatetraenoic acid (5-HPETE) and 5- hydroxyeicosatetraenoic acid (5-HETE) increased the short-circuit current (Isc) in rabbit colonic mucosa mounted in vitro in Ussing chambers. Measurements of chlorine-36 fluxes indicated that the Isc response to 5-HPETE is due to stimulation of active chlorine secretion. 9-, 11-, and 12-HPETE's and leukotrienes C4 and B4 produced either very small increases in Isc or no increase. In contrast to results in rabbit colon, no HPETE, HETE, or leukotriene was effective in rabbit ileal mucosa. The effects of 5-HPETE in the rabbit colon were unaffected by mepacrine, but could be partially blocked by indomethacin. These results suggest that drugs which block both cyclooxygenase and lipoxygenase may be effective antidiarrheals in patients with colitis.",

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T1 - Stimulation of colonic secretion by lipoxygenase metabolites of arachidonic acid

AU - Musch, Mark W.

AU - Miller, Richard J.

AU - Field, Michael

AU - Siegel, Marvin I.

PY - 1982

Y1 - 1982

N2 - Both 5-hydroperoxyeicosatetraenoic acid (5-HPETE) and 5- hydroxyeicosatetraenoic acid (5-HETE) increased the short-circuit current (Isc) in rabbit colonic mucosa mounted in vitro in Ussing chambers. Measurements of chlorine-36 fluxes indicated that the Isc response to 5-HPETE is due to stimulation of active chlorine secretion. 9-, 11-, and 12-HPETE's and leukotrienes C4 and B4 produced either very small increases in Isc or no increase. In contrast to results in rabbit colon, no HPETE, HETE, or leukotriene was effective in rabbit ileal mucosa. The effects of 5-HPETE in the rabbit colon were unaffected by mepacrine, but could be partially blocked by indomethacin. These results suggest that drugs which block both cyclooxygenase and lipoxygenase may be effective antidiarrheals in patients with colitis.

AB - Both 5-hydroperoxyeicosatetraenoic acid (5-HPETE) and 5- hydroxyeicosatetraenoic acid (5-HETE) increased the short-circuit current (Isc) in rabbit colonic mucosa mounted in vitro in Ussing chambers. Measurements of chlorine-36 fluxes indicated that the Isc response to 5-HPETE is due to stimulation of active chlorine secretion. 9-, 11-, and 12-HPETE's and leukotrienes C4 and B4 produced either very small increases in Isc or no increase. In contrast to results in rabbit colon, no HPETE, HETE, or leukotriene was effective in rabbit ileal mucosa. The effects of 5-HPETE in the rabbit colon were unaffected by mepacrine, but could be partially blocked by indomethacin. These results suggest that drugs which block both cyclooxygenase and lipoxygenase may be effective antidiarrheals in patients with colitis.

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Stimulation of colonic secretion by lipoxygenase metabolites of arachidonic acid

Abstract

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