Stirred culture of peripheral and cord blood hematopoietic cells offers advantages over traditional static systems for clinically relevant applications

Paul C. Collins, William M. Miller, E. Terry Papoutsakis*

*Corresponding author for this work

Research output: Contribution to journalArticle

73 Scopus citations

Abstract

The ability to culture hematopoietic cells in readily characterizable and scalable stirred systems, combined with the capability to utilize serum- free medium, will aid the development of clinically attractive bioreactor systems for transplantation therapies. We thus examined the proliferation and differentiation characteristics of peripheral blood (PB) mononuclear cells (MNC), cord blood (CB) MNC, and PB CD34+ cells in spinner flasks and (control) T-flask cultures in both serum-containing and serum-free media. Hematopoietic cultures initiated from all sources examined (PB MNC, CB MNC, and PB CD34+ cells) grew well in spinner vessels with either serum- containing or serum-free medium. Culture proliferation in spinner flasks was dependent on both agitator design and RPM as well as on the establishment of critical inoculum densities (ID) in both serum-containing (2 x 105 MNC/mL) and serum-free (3 x 105 MNC/mL) media. Spinner flask culture of PB MNC in serum-containing medium provided superior expansion of total cells and colony-forming cells (CFC) at high ID (1.2 x 106 cells/mL) as compared to T- flask controls. Serum-free spinner culture was comparable, if not superior, to that observed in serum-containing medium. This is the first report of stirred culture of PB or CB MNC, as well as the first report of stirred CD34+ cell culture. Additionally, this is the first account of serum-free stirred culture of hematopoietic cells from any source.

Original languageEnglish (US)
Pages (from-to)534-543
Number of pages10
JournalBiotechnology and Bioengineering
Volume59
Issue number5
DOIs
StatePublished - Sep 5 1998

Keywords

  • CD34 cells
  • Hematopoietic cell culture
  • Peripheral blood
  • Serum-free medium
  • Stirred-culture
  • Umbilical cord blood

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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