STORI Plots Enable Accurate Tracking of Individual Ion Signals

Jared O. Kafader; Steven C. Beu; Bryan P. Early; Rafael D. Melani; Kenneth R. Durbin; Vlad Zabrouskov; Alexander A. Makarov; Joshua T. Maze; Deven L. Shinholt; Ping F. Yip; Neil L. Kelleher; Philip D. Compton; Michael W. Senko

doi:10.1007/s13361-019-02309-0

STORI Plots Enable Accurate Tracking of Individual Ion Signals

Jared O. Kafader, Steven C. Beu, Bryan P. Early, Rafael D. Melani, Kenneth R. Durbin, Vlad Zabrouskov, Alexander A. Makarov, Joshua T. Maze, Deven L. Shinholt, Ping F. Yip, Neil L. Kelleher, Philip D. Compton, Michael W. Senko^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

51 Scopus citations

Abstract

Charge detection mass spectrometry (CDMS) of low-level signals is currently limited to the analysis of individual ions that generate a persistent signal during the entire observation period. Ions that disintegrate during the observation period produce reduced frequency domain signal amplitudes, which lead to an underestimation of the ion charge state, and thus the ion mass. The charge assignment can only be corrected through an accurate determination of the time of ion disintegration. The traditional mechanisms for temporal signal analysis have severe limitations for temporal resolution, spectral resolution, and signal-to-noise ratios. Selective Temporal Overview of Resonant Ions (STORI) plots provide a new framework to accurately analyze low-level time domain signals of individual ions. STORI plots allow for complete correction of intermittent signals, the differentiation of single and multiple ions at the same frequency, and the association of signals that spontaneously change frequency. [Figure not available: see fulltext.].

Original language	English (US)
Pages (from-to)	2200-2203
Number of pages	4
Journal	Journal of the American Society for Mass Spectrometry
Volume	30
Issue number	11
DOIs	https://doi.org/10.1007/s13361-019-02309-0
State	Published - Nov 1 2019

Funding

This work was funded by the Intensifying Innovation program from Thermo Fisher Scientific and was carried out in collaboration with the National Resource for Translational and Developmental Proteomics under Grant P41 GM108569 from the National Institute of General Medical Sciences, National Institutes of Health with additional support from the Sherman Fairchild Foundation. This work was funded by the Intensifying Innovation program from Thermo Fisher Scientific and was carried out in collaboration with the National Resource for Translational and Developmental Proteomics under Grant P41 GM108569 from the National Institute of General Medical Sciences, National Institutes of Health with additional support from the Sherman Fairchild Foundation.

Keywords

Charge detection mass spectrometry
FT-MS
Ion signal analysis
Orbitrap
Single ion mass spectrometry

ASJC Scopus subject areas

Structural Biology
Spectroscopy

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10.1007/s13361-019-02309-0

Cite this

Kafader, J. O., Beu, S. C., Early, B. P., Melani, R. D., Durbin, K. R., Zabrouskov, V., Makarov, A. A., Maze, J. T., Shinholt, D. L., Yip, P. F., Kelleher, N. L., Compton, P. D., & Senko, M. W. (2019). STORI Plots Enable Accurate Tracking of Individual Ion Signals. Journal of the American Society for Mass Spectrometry, 30(11), 2200-2203. https://doi.org/10.1007/s13361-019-02309-0

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title = "STORI Plots Enable Accurate Tracking of Individual Ion Signals",

abstract = "Charge detection mass spectrometry (CDMS) of low-level signals is currently limited to the analysis of individual ions that generate a persistent signal during the entire observation period. Ions that disintegrate during the observation period produce reduced frequency domain signal amplitudes, which lead to an underestimation of the ion charge state, and thus the ion mass. The charge assignment can only be corrected through an accurate determination of the time of ion disintegration. The traditional mechanisms for temporal signal analysis have severe limitations for temporal resolution, spectral resolution, and signal-to-noise ratios. Selective Temporal Overview of Resonant Ions (STORI) plots provide a new framework to accurately analyze low-level time domain signals of individual ions. STORI plots allow for complete correction of intermittent signals, the differentiation of single and multiple ions at the same frequency, and the association of signals that spontaneously change frequency. [Figure not available: see fulltext.].",

keywords = "Charge detection mass spectrometry, FT-MS, Ion signal analysis, Orbitrap, Single ion mass spectrometry",

author = "Kafader, {Jared O.} and Beu, {Steven C.} and Early, {Bryan P.} and Melani, {Rafael D.} and Durbin, {Kenneth R.} and Vlad Zabrouskov and Makarov, {Alexander A.} and Maze, {Joshua T.} and Shinholt, {Deven L.} and Yip, {Ping F.} and Kelleher, {Neil L.} and Compton, {Philip D.} and Senko, {Michael W.}",

note = "Funding Information: This work was funded by the Intensifying Innovation program from Thermo Fisher Scientific and was carried out in collaboration with the National Resource for Translational and Developmental Proteomics under Grant P41 GM108569 from the National Institute of General Medical Sciences, National Institutes of Health with additional support from the Sherman Fairchild Foundation. Funding Information: This work was funded by the Intensifying Innovation program from Thermo Fisher Scientific and was carried out in collaboration with the National Resource for Translational and Developmental Proteomics under Grant P41 GM108569 from the National Institute of General Medical Sciences, National Institutes of Health with additional support from the Sherman Fairchild Foundation. Publisher Copyright: {\textcopyright} 2019, American Society for Mass Spectrometry.",

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AU - Kafader, Jared O.

AU - Beu, Steven C.

AU - Early, Bryan P.

AU - Melani, Rafael D.

AU - Durbin, Kenneth R.

AU - Zabrouskov, Vlad

AU - Makarov, Alexander A.

AU - Maze, Joshua T.

AU - Shinholt, Deven L.

AU - Yip, Ping F.

AU - Kelleher, Neil L.

AU - Compton, Philip D.

AU - Senko, Michael W.

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N2 - Charge detection mass spectrometry (CDMS) of low-level signals is currently limited to the analysis of individual ions that generate a persistent signal during the entire observation period. Ions that disintegrate during the observation period produce reduced frequency domain signal amplitudes, which lead to an underestimation of the ion charge state, and thus the ion mass. The charge assignment can only be corrected through an accurate determination of the time of ion disintegration. The traditional mechanisms for temporal signal analysis have severe limitations for temporal resolution, spectral resolution, and signal-to-noise ratios. Selective Temporal Overview of Resonant Ions (STORI) plots provide a new framework to accurately analyze low-level time domain signals of individual ions. STORI plots allow for complete correction of intermittent signals, the differentiation of single and multiple ions at the same frequency, and the association of signals that spontaneously change frequency. [Figure not available: see fulltext.].

AB - Charge detection mass spectrometry (CDMS) of low-level signals is currently limited to the analysis of individual ions that generate a persistent signal during the entire observation period. Ions that disintegrate during the observation period produce reduced frequency domain signal amplitudes, which lead to an underestimation of the ion charge state, and thus the ion mass. The charge assignment can only be corrected through an accurate determination of the time of ion disintegration. The traditional mechanisms for temporal signal analysis have severe limitations for temporal resolution, spectral resolution, and signal-to-noise ratios. Selective Temporal Overview of Resonant Ions (STORI) plots provide a new framework to accurately analyze low-level time domain signals of individual ions. STORI plots allow for complete correction of intermittent signals, the differentiation of single and multiple ions at the same frequency, and the association of signals that spontaneously change frequency. [Figure not available: see fulltext.].

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KW - Ion signal analysis

KW - Orbitrap

KW - Single ion mass spectrometry

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STORI Plots Enable Accurate Tracking of Individual Ion Signals

Abstract

Funding

Keywords

ASJC Scopus subject areas

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