Strategies for top-down proteomics of Methanococcus jannaschii and Saccharomyces cerevisiae using a quadrupole FT-ICR-MS

Steven M. Patrie; Jay Charlebois; Derek Vander Molen; Fanyu Meng; Andrew Forbes; John P. Quinn; Christopher L. Hendrickson; Alan G. Marshall; Neil L. Kelleher

Strategies for top-down proteomics of Methanococcus jannaschii and Saccharomyces cerevisiae using a quadrupole FT-ICR-MS

Steven M. Patrie^*, Jay Charlebois, Derek Vander Molen, Fanyu Meng, Andrew Forbes, John P. Quinn, Christopher L. Hendrickson, Alan G. Marshall, Neil L. Kelleher

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

The Proteomics of methanococcus jannaschii and saccharomyces cerevisiae were studied using a quadrupole FT-ICR-MS. It was found that the top down approach fragments intact proteins within the mass spectrometer, which allowed more sequence coverage. It was shown that the micro-electrosprayed ion was desolvated and transported for accumulation either by placing the quadrupole in rf-only or mass filtered by quadrupole. Analysis shows that post transcription processing events increased proteome complexity which ultimately increased the database complexity for larger proteins in higher order organisms.

Original language	English (US)
Title of host publication	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Pages	687-688
Number of pages	2
State	Published - Dec 1 2002
Event	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: Jun 2 2002 → Jun 6 2002

Other

Other	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/Territory	United States
City	Orlando, FL
Period	6/2/02 → 6/6/02

ASJC Scopus subject areas

Spectroscopy

Cite this

@inproceedings{079ea31fda6542d08db7eb4f0cafe0da,

title = "Strategies for top-down proteomics of Methanococcus jannaschii and Saccharomyces cerevisiae using a quadrupole FT-ICR-MS",

abstract = "The Proteomics of methanococcus jannaschii and saccharomyces cerevisiae were studied using a quadrupole FT-ICR-MS. It was found that the top down approach fragments intact proteins within the mass spectrometer, which allowed more sequence coverage. It was shown that the micro-electrosprayed ion was desolvated and transported for accumulation either by placing the quadrupole in rf-only or mass filtered by quadrupole. Analysis shows that post transcription processing events increased proteome complexity which ultimately increased the database complexity for larger proteins in higher order organisms.",

author = "Patrie, {Steven M.} and Jay Charlebois and {Vander Molen}, Derek and Fanyu Meng and Andrew Forbes and Quinn, {John P.} and Hendrickson, {Christopher L.} and Marshall, {Alan G.} and Kelleher, {Neil L.}",

year = "2002",

month = dec,

day = "1",

language = "English (US)",

pages = "687--688",

booktitle = "Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics",

note = "Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics ; Conference date: 02-06-2002 Through 06-06-2002",

}

Patrie, SM, Charlebois, J, Vander Molen, D, Meng, F, Forbes, A, Quinn, JP, Hendrickson, CL, Marshall, AG & Kelleher, NL 2002, Strategies for top-down proteomics of Methanococcus jannaschii and Saccharomyces cerevisiae using a quadrupole FT-ICR-MS. in Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics. pp. 687-688, Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States, 6/2/02.

TY - GEN

T1 - Strategies for top-down proteomics of Methanococcus jannaschii and Saccharomyces cerevisiae using a quadrupole FT-ICR-MS

AU - Patrie, Steven M.

AU - Charlebois, Jay

AU - Vander Molen, Derek

AU - Meng, Fanyu

AU - Forbes, Andrew

AU - Quinn, John P.

AU - Hendrickson, Christopher L.

AU - Marshall, Alan G.

AU - Kelleher, Neil L.

PY - 2002/12/1

Y1 - 2002/12/1

N2 - The Proteomics of methanococcus jannaschii and saccharomyces cerevisiae were studied using a quadrupole FT-ICR-MS. It was found that the top down approach fragments intact proteins within the mass spectrometer, which allowed more sequence coverage. It was shown that the micro-electrosprayed ion was desolvated and transported for accumulation either by placing the quadrupole in rf-only or mass filtered by quadrupole. Analysis shows that post transcription processing events increased proteome complexity which ultimately increased the database complexity for larger proteins in higher order organisms.

AB - The Proteomics of methanococcus jannaschii and saccharomyces cerevisiae were studied using a quadrupole FT-ICR-MS. It was found that the top down approach fragments intact proteins within the mass spectrometer, which allowed more sequence coverage. It was shown that the micro-electrosprayed ion was desolvated and transported for accumulation either by placing the quadrupole in rf-only or mass filtered by quadrupole. Analysis shows that post transcription processing events increased proteome complexity which ultimately increased the database complexity for larger proteins in higher order organisms.

UR - http://www.scopus.com/inward/record.url?scp=2442711603&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2442711603&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:2442711603

SP - 687

EP - 688

BT - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

T2 - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

Y2 - 2 June 2002 through 6 June 2002

ER -

Strategies for top-down proteomics of Methanococcus jannaschii and Saccharomyces cerevisiae using a quadrupole FT-ICR-MS

Abstract

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ASJC Scopus subject areas

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