Strategies to regulate transcription factor-mediated gene positioning and interchromosomal clustering at the nuclear periphery

Carlo Randise-Hinchliff, Robert Coukos, Varun Sood, Michael Chas Sumner, Stefan Zdraljevic, Lauren Meldi Sholl, Donna Garvey Brickner, Sara Ahmed, Lauren Watchmaker, Jason H. Brickner*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

In budding yeast, targeting of active genes to the nuclear pore complex (NPC) and interchromosomal clustering is mediated by transcription factor (TF) binding sites in the gene promoters. For example, the binding sites for the TFs Put3, Ste12, and Gcn4 are necessary and sufficient to promote positioning at the nuclear periphery and interchromosomal clustering. However, in all three cases, gene positioning and interchromosomal clustering are regulated. Under uninducing conditions, local recruitment of the Rpd3(L) histone deacetylase by transcriptional repressors blocks Put3 DNA binding. This is a general function of yeast repressors: 16 of 21 repressors blocked Put3-mediated subnuclear positioning; 11 of these required Rpd3. In contrast, Ste12-mediated gene positioning is regulated independently of DNA binding by mitogen-activated protein kinase phosphorylation of the Dig2 inhibitor, and Gcn4-dependent targeting is up-regulated by increasing Gcn4 protein levels. These different regulatory strategies provide either qualitative switch-like control or quantitative control of gene positioning over different time scales.

Original languageEnglish (US)
Pages (from-to)633-646
Number of pages14
JournalJournal of Cell Biology
Volume212
Issue number6
DOIs
StatePublished - Mar 16 2016

ASJC Scopus subject areas

  • Cell Biology

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