Strategy for distinguishing a new DQB 1 allele (DQB1(*)0611) from the closely related DQB 1(*)0602 allele via sequence specific PCR or direct DNA sequencing

T. M. Williams*, S. Bassinger, C. Moehlenkamp, J. Wu, G. D. Montoya, B. B. Griffith, J. D. McAuley, S. Goldman, D. H. Maurer, G. M. Troup

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

A novel DQ6 allele (DQB 1(*)0611) was identified via direct DNA sequencing in an African-American donor for bone marrow transplantation. The allele was not suspected on the basis of a sequence specific PCR assay which instead indicated the presence of DQB1(*)0602. DQB1(*)0602 and DQB1(*)0611 differ in exon 2 only at codon 9 resulting in a tyrosine substitution for phenylalanine. A modification of current DQB 1 sequence specific PCR assays was devised which allows distinction between the closely related DQB1(*)0602 and DQB1(*)0611 alleles. Preliminary allele frequency studies suggest that DQB1(*)0611 is rare both in a non-African American sample and in Americans of African descent carrying DR11, DQ6 haplotypes. The selection of various DQB1(*)0611 detection methods is discussed.

Original languageEnglish (US)
Pages (from-to)143-147
Number of pages5
JournalTissue Antigens
Volume48
Issue number3
DOIs
StatePublished - Jan 1 1996

Keywords

  • Bone marrow transplantation
  • Direct DNA sequencing
  • HLA-DQB1
  • Sequence specific PCR

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Biochemistry
  • Genetics

Fingerprint Dive into the research topics of 'Strategy for distinguishing a new DQB 1 allele (DQB1(*)0611) from the closely related DQB 1(*)0602 allele via sequence specific PCR or direct DNA sequencing'. Together they form a unique fingerprint.

Cite this