Stretch-induced activation of AMP kinase in the lung requires dystroglycan

G. R.Scott Budinger, Daniela Urich, Phillip J. DeBiase, Sergio E. Chiarella, Zachary O. Burgess, Christina M. Baker, Saul Soberanes, Gokhän M. Mutlu, Jonathan C.R. Jones

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

Lung cells are exposed to cyclic stretch during normal respiration and during positive pressure mechanical ventilation administered to support gas exchange. Dystroglycan is a ubiquitously expressed matrix receptor that is required for normal basement membrane formation during embryogenesis and for maintaining the function of skeletal muscle myocytes and neurons where it links cells to matrix. We previously reported that equibiaxial stretch of primary alveolar epithelial cells activated the MAP kinase pathway ERK1/2 through a mechanism that required an interaction between dystroglycan and matrix. We determined whether this mechanism of mechanotransduction activates other signaling cascades in lung epithelium. Exposure of rat epithelial alveolar type II cells (AEC) to cyclic mechanical stretch resulted in activation of 5′ AMP-activated protein kinase (AMPK). This response was not affected by pretreatment of AEC with the ERK inhibitor PD98059 but was inhibited by knockdown in dystroglycan expression. Moreover, production of reactive oxygen species was enhanced in mechanically stimulated AEC in which dystroglycan was knocked down. This enhancement was reversed by treatment of AEC with an AMPK activator. Activation of AMPK was also observed in lung homogenates from mice after 15 minutes of noninjurious mechanical ventilation. Furthermore, knockdown of dystroglycan in the lungs of mice using an adenovirus encoding a dystroglycan shRNA prevented the stretch-induced activation of AMPK. These results suggest that exposure to cyclic stretch activates the metabolic sensing pathway AMPK in the lung epithelium and supports a novel role for dystroglycan in this mechanotransduction.

Original languageEnglish (US)
Pages (from-to)666-672
Number of pages7
JournalAmerican journal of respiratory cell and molecular biology
Volume39
Issue number6
DOIs
StatePublished - Dec 1 2008

Keywords

  • Acute respiratory distress syndrome
  • Lung injury
  • Mechanical ventilation
  • Stretch

ASJC Scopus subject areas

  • Molecular Biology
  • Pulmonary and Respiratory Medicine
  • Clinical Biochemistry
  • Cell Biology

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    Budinger, G. R. S., Urich, D., DeBiase, P. J., Chiarella, S. E., Burgess, Z. O., Baker, C. M., Soberanes, S., Mutlu, G. M., & Jones, J. C. R. (2008). Stretch-induced activation of AMP kinase in the lung requires dystroglycan. American journal of respiratory cell and molecular biology, 39(6), 666-672. https://doi.org/10.1165/rcmb.2007-0432OC