Abstract
Objective: In endometrium, stromal progesterone receptors mediate production of paracrine factors, which enhance binding of the transcription factor specific protein-1 to the promoter of the gene encoding the 17β-hydroxysteroid dehydrogenase type 2 enzyme responsible for converting biologically active estradiol to estrone in epithelium. The objective of this study is to define the cellular defect responsible for the disruption of this stromal-epithelial interaction in endometriosis. Study Design: We determined the effects of conditioned media generated from primary human eutopic endometrial stromal cells vs endometriotic stromal cells on Ishikawa malignant endometrial epithelial cells. Results: Conditioned media from progestin-pretreated eutopic endometrial stromal cells but not edometriotic stromal cells significantly stimulated specific protein-1 protein levels, 17β-hydroxysteroid dehydrogenase type 2 messenger RNA levels and promoter activity, and binding activity of specific protein-1 to the 17β-hydroxysteroid dehydrogenase type 2 promoter region in Ishikawa cells. Conclusion: A stromal cell defect in endometriosis blocks formation of progesterone-dependent production of factors leading to 17β-hydroxysteroid dehydrogenase type 2 deficiency and defective conversion of estradiol to estrone in epithelium.
Original language | English (US) |
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Pages (from-to) | 391.e1-391.e8 |
Journal | American journal of obstetrics and gynecology |
Volume | 196 |
Issue number | 4 |
DOIs | |
State | Published - Apr 2007 |
Funding
This study was supported by NIH grant HD40093 and a grant from Friends of Prentice.
Keywords
- 17β-hydroxysteroid dehydrogenase type 2
- R5020
- conditioned medium
- endometriosis
- endometrium
- epithelium
- estradiol
- estrone
- paracrine
- progesterone receptor
- specific protein-1
- stroma
ASJC Scopus subject areas
- Obstetrics and Gynecology