Structural and elemental changes in glioblastoma cells: In situ: Complementary imaging with high resolution visible light- and X-ray microscopy

Tanja Dučić*, Tatjana Paunesku, Si Chen, Milena Ninković, Swetlana Speling, Charlene Wilke, Barry Lai, Gayle E Woloschak

*Corresponding author for this work

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

The glioblastoma (GBM) is characterized by a short median survival and an almost 100% tumor related mortality. GBM cells exhibit highly invasive behavior whose mechanisms are not yet fully understood. The present study explores application of X-ray and visible light microscopy to display the elemental and structural images of cells from 3 patient derived GMB samples and an established GMB cell line. Slight differences in elemental concentrations, in actin cytoskeleton organization and cell morphology were noted between all cells types by X-ray fluorescence and full field soft X-ray microscopy, as well as the Structured Illumination Super-resolution Microscope (SIM). Different sample preparation approaches were used to match each imaging technique. While preparation for SIM included cell fixation and staining, intact frozen hydrated cells were used for the trace element imaging by hard X-ray fluorescence and exploration of the structural features by soft X-ray absorption tomography. Each technique documented differences between samples with regard to morphology and elemental composition and underscored the importance of use of multiple patient derived samples for detailed GBM study.

Original languageEnglish (US)
Pages (from-to)356-365
Number of pages10
JournalAnalyst
Volume142
Issue number2
DOIs
StatePublished - Jan 21 2017

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

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