Structural and functional analysis of betaine aldehyde dehydrogenase from Staphylococcus aureus

Andrei S. Halavaty, Rebecca L. Rich, Chao Chen, Jeong Chan Joo, George Minasov, Ievgeniia Dubrovska, James R. Winsor, David G. Myszka, Mark Duban, Ludmilla Shuvalova, Alexander F. Yakunin, Wayne F. Anderson*

*Corresponding author for this work

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

When exposed to high osmolarity, methicillin-resistant Staphylococcus aureus (MRSA) restores its growth and establishes a new steady state by accumulating the osmoprotectant metabolite betaine. Effective osmoregulation has also been implicated in the acquirement of a profound antibiotic resistance by MRSA. Betaine can be obtained from the bacterial habitat or produced intracellularly from choline via the toxic betaine aldehyde (BA) employing the choline dehydrogenase and betaine aldehyde dehydrogenase (BADH) enzymes. Here, it is shown that the putative betaine aldehyde dehydrogenase SACOL2628 from the early MRSA isolate COL (SaBADH) utilizes betaine aldehyde as the primary substrate and nicotinamide adenine dinucleotide (NAD+) as the cofactor. Surface plasmon resonance experiments revealed that the affinity of NAD+, NADH and BA for SaBADH is affected by temperature, pH and buffer composition. Five crystal structures of the wild type and three structures of the Gly234Ser mutant of SaBADH in the apo and holo forms provide details of the molecular mechanisms of activity and substrate specificity/inhibition of this enzyme.

Original languageEnglish (US)
Pages (from-to)1159-1175
Number of pages17
JournalActa Crystallographica Section D: Biological Crystallography
Volume71
DOIs
StatePublished - May 1 2015

Keywords

  • Staphylococcus aureus
  • betaine aldehyde dehydrogenase
  • high-throughput approach
  • infectious diseases
  • structural genomics

ASJC Scopus subject areas

  • Structural Biology

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