Structural and functional analysis of the SET3 histone deacetylase complex

Alexis A. Reyes, Susan Fishbain, Yuan He*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


The SET3 complex (SET3C) is a seven-subunit histone deacetylase complex that is capable of transcriptional regulation. Methylated histone 3 marks recruit SET3C to the nucleosome, and the SET3C catalytic subunits deacetylate the histone 3 and 4 tails. There is very limited structural knowledge of the SET3C subunits, with most subunits having unknown structures or functions. Here, a catalytically active SET3 complex was endogenously purified from Saccharomyces cerevisiae and utilized for negative-stain electron microscopy (EM) to determine an apo model for the holo complex. The negative-stain EM 3D model revealed a three-lobe architecture, with each lobe extending from a central point.

Original languageEnglish (US)
Pages (from-to)113-118
Number of pages6
JournalActa Crystallographica Section F: Structural Biology Communications
StatePublished - Mar 1 2022


  • SET3 complex
  • electron microscopy
  • histone deacetylation

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Genetics
  • Biophysics
  • Structural Biology
  • Biochemistry


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