Structural and Viscoelastic Properties of Actin Networks Formed by Espin or Pathologically Relevant Espin Mutants

Oliver Lieleg, Kurt M. Schmoller, Kirstin R. Purdy Drew, Mireille M.A.E. Claessens, Christine Semmrich, Lili Zheng, James R. Bartles, Andreas R. Bausch

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

The structural organization of the cytoskeleton determines its viscoelastic response which is crucial for the correct functionality of living cells. Both the mechanical response and microstructure of the cytoskeleton are regulated on a microscopic level by the local activation of different actin binding and/or bundling proteins (ABPs). Misregulations in the expression of these ABPs or mutations in their sequence can entail severe cellular dysfunctions and diseases. Here, we study the structural and viscoelastic properties of reconstituted actin networks cross-linked by the ABP espin and compare the obtained network properties to those of other bundled actin networks. Moreover, we quantify the impact of pathologically relevant espin mutations on the viscoelastic properties of these cytoskeletal networks.

Original languageEnglish (US)
Pages (from-to)2813-2817
Number of pages5
JournalChemPhysChem
Volume10
Issue number16
DOIs
StatePublished - Nov 9 2009

Keywords

  • Actin
  • Biophysics
  • Fluorescence
  • Mutation
  • Proteins

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Physical and Theoretical Chemistry

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    Lieleg, O., Schmoller, K. M., Purdy Drew, K. R., Claessens, M. M. A. E., Semmrich, C., Zheng, L., Bartles, J. R., & Bausch, A. R. (2009). Structural and Viscoelastic Properties of Actin Networks Formed by Espin or Pathologically Relevant Espin Mutants. ChemPhysChem, 10(16), 2813-2817. https://doi.org/10.1002/cphc.200900604