Structural basis of FFAT motif-mediated ER targeting

Stephen E. Kaiser, Jason H. Brickner, Amy R. Reilein, Tim D. Fenn, Peter Walter, Axel T. Brunger*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

151 Scopus citations

Abstract

The FFAT motif is a targeting signal responsible for localizing a number of proteins to the cytosolic surface of the endoplasmic reticulum (ER) and to the nuclear membrane. FFAT motifs bind to members of the highly conserved VAP protein family, which are tethered to the cytoplasmic face of the ER by a C-terminal transmembrane domain. We have solved crystal structures of the rat VAP-A MSP homology domain alone and in complex with an FFAT motif. The co-crystal structure was used to design a VAP mutant that disrupts rat and yeast VAP-FFAT interactions in vitro. The FFAT binding-defective mutant also blocked function of the VAP homolog Scs2p in yeast. Finally, overexpression of the FFAT binding-defective VAP in COS7 cells dramatically altered ER morphology. Our data establish the structural basis of FFAT-mediated ER targeting and suggest that FFAT-targeted proteins play an important role in determining ER morphology.

Original languageEnglish (US)
Pages (from-to)1035-1045
Number of pages11
JournalStructure
Volume13
Issue number7
DOIs
StatePublished - Jul 2005

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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