Structural model of a complex between the heterotrimeric G protein, Gsα, and tubulin

Brian T. Layden, Witchuda Saengsawang, Robert J. Donati, Shuo Yang, Debbie C. Mulhearn, Michael E. Johnson, Mark M. Rasenick*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

A number of studies have demonstrated interplay between the cytoskeleton and G protein signaling. Many of these studies have determined a specific interaction between tubulin, the building block of microtubules, and G proteins. The α subunits of some heterotrimeric G proteins, including Gsα, have been shown to interact strongly with tubulin. Binding of Gα to tubulin results in increased dynamicity of microtubules due to activation of GTPase of tubulin. Tubulin also activates Gsα via a direct transfer of GTP between these molecules. Structural insight into the interaction between tubulin and Gsα was required, and was determined, in this report, through biochemical and molecular docking techniques. Solid phase peptide arrays suggested that a portion of the amino terminus, α2-β4 (the region between switch II and switch III) and α3-β5 (just distal to the switch III region) domains of Gsα are important for interaction with tubulin. Molecular docking studies revealed the best-fit models based on the biochemical data, showing an interface between the two molecules that includes the adenylyl cyclase/Gβγ interaction regions of Gsα and the exchangeable nucleotide-binding site of tubulin. These structural models explain the ability of tubulin to facilitate GTP exchange on Gα and the ability of Gα to activate tubulin GTPase.

Original languageEnglish (US)
Pages (from-to)964-973
Number of pages10
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume1783
Issue number6
DOIs
StatePublished - Jun 2008

Keywords

  • Crystal structure
  • Cytoskeleton
  • G protein
  • GTPase
  • Microtubule

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Structural model of a complex between the heterotrimeric G protein, Gsα, and tubulin'. Together they form a unique fingerprint.

Cite this