Abstract
The paramyxovirus hemagglutinin-neuraminidase (HN) functions in virus attachment to cells, cleavage of sialic acid from oligosaccharides, and stimulating membrane fusion during virus entry into cells. The structural basis for these diverse functions remains to be fully understood. We report the crystal structures of the parainfluenza virus 5 (SV5) HN and its complexes with sialic acid, the inhibitor DANA, and the receptor sialyllactose. SV5 HN shares common structural features with HN of Newcastle disease virus (NDV) and human parainfluenza 3 (HPIV3), but unlike the previously determined HN structures, the SV5 HN forms a tetramer in solution, which is thought to be the physiological oligomer. The sialyllactose complex reveals intact receptor within the active site, but no major conformational changes in the protein. The SV5 HN structures do not support previously proposed models for HN action in membrane fusion and suggest alternative mechanisms by which HN may promote virus entry into cells.
Original language | English (US) |
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Pages (from-to) | 803-815 |
Number of pages | 13 |
Journal | Structure |
Volume | 13 |
Issue number | 5 |
DOIs | |
State | Published - May 2005 |
Funding
This research was supported in part by National Institutes of Health research grants GM-61050 (T.S.J.) and AI-23173 (R.A.L.). R.A.L. is an investigator of the Howard Hughes Medical Institute.
ASJC Scopus subject areas
- Structural Biology
- Molecular Biology
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Dive into the research topics of 'Structural studies of the parainfluenza virus 5 hemagglutinin-neuraminidase tetramer in complex with its receptor, sialyllactose'. Together they form a unique fingerprint.Datasets
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Parainfluenza Virus 5 (SV5) Hemagglutinin-Neuraminidase (HN) (pH 8.0)
Yuan, P. (Contributor), Thompson, T. B. (Contributor), Wurzburg, B. A. (Contributor), Paterson, R. G. (Contributor), Lamb, R. A. (Contributor) & Jardetzky, T. S. (Contributor), Protein Data Bank (PDB), May 24 2005
DOI: 10.2210/pdb1Z4Y/pdb, https://www.wwpdb.org/pdb?id=pdb_00001z4y
Dataset
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Parainfluenza Virus 5 (SV5) Hemagglutinin-Neuraminidase (HN) with ligand DANA (soaked with sialic acid, pH 8.0)
Yuan, P. (Contributor), Thompson, T. B. (Contributor), Wurzburg, B. A. (Contributor), Paterson, R. G. (Contributor), Lamb, R. A. (Contributor) & Jardetzky, T. S. (Contributor), Protein Data Bank (PDB), May 24 2005
DOI: 10.2210/pdb1Z50/pdb, https://www.wwpdb.org/pdb?id=pdb_00001z50
Dataset
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Parainfluenza Virus 5 (SV5) Hemagglutinin-Neuraminidase (HN) with ligand DANA (soaked with DANA, pH8.0)
Yuan, P. (Contributor), Thompson, T. B. (Contributor), Wurzburg, B. A. (Contributor), Paterson, R. G. (Contributor), Lamb, R. A. (Contributor) & Jardetzky, T. S. (Contributor), Protein Data Bank (PDB), May 24 2005
DOI: 10.2210/pdb1Z4W/pdb, https://www.wwpdb.org/pdb?id=pdb_00001z4w
Dataset