Structural transitions of a twisted and stretched DNA molecule

J. F. Léger; G. Romano; A. Sarkar; J. Robert; L. Bourdieu; D. Chatenay; J. F. Marko

doi:10.1103/PhysRevLett.83.1066

Structural transitions of a twisted and stretched DNA molecule

J. F. Léger, G. Romano, A. Sarkar, J. Robert, L. Bourdieu, D. Chatenay, J. F. Marko

Molecular Biosciences

Research output: Contribution to journal › Article › peer-review

263 Scopus citations

Abstract

We report results of a micromanipulation study of single double-helical DNA molecules at forces up to 150 pN. Depending on whether the DNA winding is allowed to relax, or held fixed, qualitatively different structural transitions are observed. By studying the transitions as a function of winding the different DNA structures underlying them are characterized; this allows us to report the first estimate of S-DNA helicity. A model is introduced to describe these transitions; in addition to B-DNA, we find that four DNA states are needed to describe the experiments.

Original language	English (US)
Pages (from-to)	1066-1069
Number of pages	4
Journal	Physical review letters
Volume	83
Issue number	5
DOIs	https://doi.org/10.1103/PhysRevLett.83.1066
State	Published - Jan 1 1999

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General Physics and Astronomy

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AU - Romano, G.

AU - Sarkar, A.

AU - Robert, J.

AU - Bourdieu, L.

AU - Chatenay, D.

AU - Marko, J. F.

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AB - We report results of a micromanipulation study of single double-helical DNA molecules at forces up to 150 pN. Depending on whether the DNA winding is allowed to relax, or held fixed, qualitatively different structural transitions are observed. By studying the transitions as a function of winding the different DNA structures underlying them are characterized; this allows us to report the first estimate of S-DNA helicity. A model is introduced to describe these transitions; in addition to B-DNA, we find that four DNA states are needed to describe the experiments.

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Structural transitions of a twisted and stretched DNA molecule

Abstract

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