Structure-function analysis of SAP97, a modular scaffolding protein that drives dendrite growth

L. Zhang, F. C. Hsu, J. Mojsilovic-Petrovic, A. M. Jablonski, J. Zhai, D. A. Coulter, R. G. Kalb*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

14 Scopus citations


Activation of AMPA receptors assembled with the GluA1 subunit can promote dendrite growth in a manner that depends on its direct binding partner, SAP97. SAP97 is a modular scaffolding protein that has at least seven recognizable protein-protein interaction domains. Several complementary approaches were employed to show that the dendrite branching promoting action of full length SAP97 depends on ligand(s) that bind to the PDZ3 domain. Ligand(s) to PDZ1, PDZ2 and I3 domains also contribute to dendrite growth. The ability of PDZ3 ligand(s) to promote dendrite growth depends on localization at the plasma membrane along with GluA1 and SAP97. These results suggest that the assembly of a multi-protein complex at or near synapses is vital for the translation of AMPA-R activity into dendrite growth.

Original languageEnglish (US)
Pages (from-to)31-44
Number of pages14
JournalMolecular and Cellular Neuroscience
StatePublished - Mar 1 2015


  • GluA1
  • PDZ domain
  • Spinal cord neuron

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Molecular Biology
  • Cell Biology


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