Structure of a new crystal form of human Hsp70 ATPase domain

Jerzy Osipiuk, Martin A. Walsh, Brian C. Freeman, Richard I Morimoto, Andrzej Joachimiak*

*Corresponding author for this work

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

Hsp70 proteins are highly conserved proteins induced by heat shock and other stress conditions. An ATP-binding domain of human Hsp70 protein has been crystallized in two major morphological forms at pH 7.0 in the presence of PEG 8000 and CaCl2. Both crystal forms belong to the orthorhombic space group P212121, but show no resemblance in unit-cell parameters. Analysis of the crystal structures for both forms shows a 1-2 Å shift of one of the subdomains of the protein. This conformational change could reflect a 'natural' flexibility of the protein which might be relevant to ATP binding and may facilitate the interaction of other proteins with Hsp70 protein.

Original languageEnglish (US)
Pages (from-to)1105-1107
Number of pages3
JournalActa Crystallographica Section D: Biological Crystallography
Volume55
Issue number5
DOIs
StatePublished - May 1 1999

ASJC Scopus subject areas

  • Structural Biology

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