Structure of a new neurotoxin from the scorpion Buthus martensii Karsch at 1.76 Å

Xiao Lin He, Jun Peng Deng, Miao Wang, Ying Zhang, Da Cheng Wang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

A new neurotoxin BmK M2, toxic to both mammals and insects, with the strongest toxicity in the BmK toxin series, has been purified from the Chinese scorpion Buthus martensii Karsch and crystallized with MPD at pH 7.5. The crystals are orthorhombic, belonging to space group P212121, with unit-cell parameters a = 36.64, b = 36.95, c = 37.23 Å. The structure was solved by molecular replacement and refined to R = 0.186 for all reflections to a resolution of 1.76 Å. The whole sequence (64 residues) of BmK M2 was determined by crystallographic analysis based on high-resolution data and the homologous model of BmK M8. The refined BmK M2 structure shows a non-proline cis peptide bond between Pro9 and His10 which enables the C-terminal segment to adopt a conformation different to that of the weak toxin BmK M8. Recently, a mutation analysis had suggested that both the tenth residue and the C-terminus play key roles in receptor binding. Therefore, these features may be related to the binding selectivity of the group III α-like toxins. The charge changes of residues 8, 10, 18, 28, 55 and 59 from neutral or negative to positive or neutral, which leads to a positive electrostatic potential surface, may be responsible for the high toxicity of BmK M2.

Original languageEnglish (US)
Pages (from-to)25-33
Number of pages9
JournalActa Crystallographica Section D: Biological Crystallography
Volume56
Issue number1
DOIs
StatePublished - Jan 1 2000

ASJC Scopus subject areas

  • Structural Biology

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