@article{0606121ad3d24171922a89941004e14d,
title = "Structure of the Bacillus anthracis dTDP-L-rhamnose biosynthetic pathway enzyme: dTDP-α-D-glucose 4,6-dehydratase, RfbB",
abstract = "Many bacteria require L-rhamnose as a key cell wall component. This sugar is transferred to the cell wall using an activated donor dTDP-L-rhamnose, which is produced by the dTDP-L-rhamnose biosynthetic pathway. We determined the crystal structure of the second enzyme of this pathway dTDP-α-D-glucose 4,6-dehydratase (RfbB) from Bacillus anthracis. Interestingly, RfbB only crystallized in the presence of the third enzyme of the pathway RfbC; however, RfbC was not present in the crystal. Our work represents the first complete structural characterization of the four proteins of this pathway in a single Gram-positive bacterium.",
keywords = "Cell wall polysaccharide producing enzymes, Co-crystallization of metabolic enzymes, Dehydratase, Protein crystallization, Therapeutic drug target",
author = "Trevor Gokey and Halavaty, {Andrei S.} and George Minasov and Anderson, {Wayne F.} and Kuhn, {Misty L.}",
note = "Funding Information: This project was funded in part with Startup Funds from San Francisco State University (to MLK). Additional funding for this project included Federal funds from the National Institute of Allergy and Infectious Diseases , National Institutes of Health (NIH), U.S. Department of Health and Human Services , under Contracts No. HHSN272200700058C and HHSN272201200026C (to WFA). Funding Information: This project was funded in part with Startup Funds from San Francisco State University (to MLK). Additional funding for this project included Federal funds from the National Institute of Allergy and Infectious Diseases, National Institutes of Health (NIH), U.S. Department of Health and Human Services, under Contracts No. HHSN272200700058C and HHSN272201200026C (to WFA). Publisher Copyright: {\textcopyright} 2018 Elsevier Inc.",
year = "2018",
month = may,
doi = "10.1016/j.jsb.2018.01.006",
language = "English (US)",
volume = "202",
pages = "175--181",
journal = "Journal of Structural Biology",
issn = "1047-8477",
publisher = "Academic Press Inc.",
number = "2",
}