Structure of the Bacillus anthracis dTDP-L-rhamnose biosynthetic pathway enzyme: dTDP-α-D-glucose 4,6-dehydratase, RfbB

Trevor Gokey; Andrei S. Halavaty; George Minasov; Wayne F. Anderson; Misty L. Kuhn

doi:10.1016/j.jsb.2018.01.006

Structure of the Bacillus anthracis dTDP-L-rhamnose biosynthetic pathway enzyme: dTDP-α-D-glucose 4,6-dehydratase, RfbB

Trevor Gokey, Andrei S. Halavaty, George Minasov, Wayne F. Anderson, Misty L. Kuhn^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Many bacteria require L-rhamnose as a key cell wall component. This sugar is transferred to the cell wall using an activated donor dTDP-L-rhamnose, which is produced by the dTDP-L-rhamnose biosynthetic pathway. We determined the crystal structure of the second enzyme of this pathway dTDP-α-D-glucose 4,6-dehydratase (RfbB) from Bacillus anthracis. Interestingly, RfbB only crystallized in the presence of the third enzyme of the pathway RfbC; however, RfbC was not present in the crystal. Our work represents the first complete structural characterization of the four proteins of this pathway in a single Gram-positive bacterium.

Original language	English (US)
Pages (from-to)	175-181
Number of pages	7
Journal	Journal of Structural Biology
Volume	202
Issue number	2
DOIs	https://doi.org/10.1016/j.jsb.2018.01.006
State	Published - May 2018

Funding

This project was funded in part with Startup Funds from San Francisco State University (to MLK). Additional funding for this project included Federal funds from the National Institute of Allergy and Infectious Diseases , National Institutes of Health (NIH), U.S. Department of Health and Human Services , under Contracts No. HHSN272200700058C and HHSN272201200026C (to WFA). This project was funded in part with Startup Funds from San Francisco State University (to MLK). Additional funding for this project included Federal funds from the National Institute of Allergy and Infectious Diseases, National Institutes of Health (NIH), U.S. Department of Health and Human Services, under Contracts No. HHSN272200700058C and HHSN272201200026C (to WFA).

Keywords

Cell wall polysaccharide producing enzymes
Co-crystallization of metabolic enzymes
Dehydratase
Protein crystallization
Therapeutic drug target

ASJC Scopus subject areas

Structural Biology

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10.1016/j.jsb.2018.01.006

Cite this

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title = "Structure of the Bacillus anthracis dTDP-L-rhamnose biosynthetic pathway enzyme: dTDP-α-D-glucose 4,6-dehydratase, RfbB",

abstract = "Many bacteria require L-rhamnose as a key cell wall component. This sugar is transferred to the cell wall using an activated donor dTDP-L-rhamnose, which is produced by the dTDP-L-rhamnose biosynthetic pathway. We determined the crystal structure of the second enzyme of this pathway dTDP-α-D-glucose 4,6-dehydratase (RfbB) from Bacillus anthracis. Interestingly, RfbB only crystallized in the presence of the third enzyme of the pathway RfbC; however, RfbC was not present in the crystal. Our work represents the first complete structural characterization of the four proteins of this pathway in a single Gram-positive bacterium.",

keywords = "Cell wall polysaccharide producing enzymes, Co-crystallization of metabolic enzymes, Dehydratase, Protein crystallization, Therapeutic drug target",

author = "Trevor Gokey and Halavaty, {Andrei S.} and George Minasov and Anderson, {Wayne F.} and Kuhn, {Misty L.}",

note = "Funding Information: This project was funded in part with Startup Funds from San Francisco State University (to MLK). Additional funding for this project included Federal funds from the National Institute of Allergy and Infectious Diseases , National Institutes of Health (NIH), U.S. Department of Health and Human Services , under Contracts No. HHSN272200700058C and HHSN272201200026C (to WFA). Funding Information: This project was funded in part with Startup Funds from San Francisco State University (to MLK). Additional funding for this project included Federal funds from the National Institute of Allergy and Infectious Diseases, National Institutes of Health (NIH), U.S. Department of Health and Human Services, under Contracts No. HHSN272200700058C and HHSN272201200026C (to WFA). Publisher Copyright: {\textcopyright} 2018 Elsevier Inc.",

year = "2018",

month = may,

doi = "10.1016/j.jsb.2018.01.006",

language = "English (US)",

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AU - Halavaty, Andrei S.

AU - Minasov, George

AU - Anderson, Wayne F.

AU - Kuhn, Misty L.

N1 - Funding Information: This project was funded in part with Startup Funds from San Francisco State University (to MLK). Additional funding for this project included Federal funds from the National Institute of Allergy and Infectious Diseases , National Institutes of Health (NIH), U.S. Department of Health and Human Services , under Contracts No. HHSN272200700058C and HHSN272201200026C (to WFA). Funding Information: This project was funded in part with Startup Funds from San Francisco State University (to MLK). Additional funding for this project included Federal funds from the National Institute of Allergy and Infectious Diseases, National Institutes of Health (NIH), U.S. Department of Health and Human Services, under Contracts No. HHSN272200700058C and HHSN272201200026C (to WFA). Publisher Copyright: © 2018 Elsevier Inc.

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N2 - Many bacteria require L-rhamnose as a key cell wall component. This sugar is transferred to the cell wall using an activated donor dTDP-L-rhamnose, which is produced by the dTDP-L-rhamnose biosynthetic pathway. We determined the crystal structure of the second enzyme of this pathway dTDP-α-D-glucose 4,6-dehydratase (RfbB) from Bacillus anthracis. Interestingly, RfbB only crystallized in the presence of the third enzyme of the pathway RfbC; however, RfbC was not present in the crystal. Our work represents the first complete structural characterization of the four proteins of this pathway in a single Gram-positive bacterium.

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KW - Protein crystallization

KW - Therapeutic drug target

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Structure of the Bacillus anthracis dTDP-L-rhamnose biosynthetic pathway enzyme: dTDP-α-D-glucose 4,6-dehydratase, RfbB

Abstract

Funding

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

2.9 Angstrom Resolution Crystal Structure of dTDP-Glucose 4,6-dehydratase (rfbB) from Bacillus anthracis str. Ames in Complex with NAD.

Cite this

Structure of the Bacillus anthracis dTDP-L-rhamnose biosynthetic pathway enzyme: dTDP-α-D-glucose 4,6-dehydratase, RfbB

Abstract

Funding

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Datasets

2.9 Angstrom Resolution Crystal Structure of dTDP-Glucose 4,6-dehydratase (rfbB) from Bacillus anthracis str. Ames in Complex with NAD.

Cite this