Struktura tsitoskeleta myshinykh émbrional'nykh fibroblastov. Elektronno-mikroskopicheskoe issledovanie platinovykh replik.

Translated title of the contribution: Structure of the cytoskeleton of embryonic muscle fibroblasts. Electron microscopic study of platinum replicas

T. M. Svitkina*, A. A. Shevelev, A. D. Bershadskiǐ, V. I. Gel'fand

*Corresponding author for this work

Research output: Contribution to journalReview article

2 Scopus citations

Abstract

Cultured mouse embryo fibroblasts were extracted with 1% Triton X-100 at pH 6.7 in buffer containing EGTA and stabilizing supplements. Exposed during this extraction cytoskeletons were fixed, dried with the critical point technique, and shadowed with platinum. The platinum replicas of cytoskeleton were used for electron microscopic characterization of the three-dimensional distribution of cytoskeletal fibrils in cytoplasm. Four cytoplasmic regions are revealed with different cytoskeletal structure; these being a "mesh-work zone", a "loose zone", which together formed an active edge of cell lamelloplasm, the "lamella proper" and endoplasm. The two former zones are occupied with dense three-dimensional or loose planar actin network, respectively. The "lamella proper" contains two-dimensional network of different fibrils: microfilaments, microtubules, intermediate filaments and thin connective filaments. The central perinuclear area has a thick microfilament sheath at dorsal cell surface. The most important cytoplasmic elements of fibroblasts are actin microfilament bundles. The fine structure of these bundles, their junctions with each other ("organizing centers") and their terminal parts corresponding to cell-substrate focal contacts are described.

Translated title of the contributionStructure of the cytoskeleton of embryonic muscle fibroblasts. Electron microscopic study of platinum replicas
Original languageRussian
Pages (from-to)1004-1012
Number of pages9
JournalTsitologiya
Volume25
Issue number9
StatePublished - Sep 1 1983

ASJC Scopus subject areas

  • Medicine(all)

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