Structure of the lysozyme from bacteriophage T4: An electron density map at 2.4 Å resolution

S. J. Remington; W. F. Anderson; J. Owen; L. F Ten Eyck; C. T. Grainger; B. W. Matthews

doi:10.1016/0022-2836(78)90245-0

Structure of the lysozyme from bacteriophage T4: An electron density map at 2.4 Å resolution

S. J. Remington^*, W. F. Anderson, J. Owen, L. F Ten Eyck, C. T. Grainger, B. W. Matthews

^*Corresponding author for this work

Biochemistry and Molecular Genetics

Research output: Contribution to journal › Article › peer-review

118 Scopus citations

Abstract

The three-dimensional structure of the lysozyme from bacteriophage T4 has been determined from a 2.4 Å resolution electron density map. The map, determined using isomorphous replacement and anomalous scattering differences from platinum and mercury-substituted crystals, reveals the approximate conformation of the molecule unambiguously. Details of the structure determination are presented and the conformation of the molecule is described in detail. The observed properties of those mutant lysozymes for which the changes in the amino acid sequence have been determined are discussed in the light of the three-dimensional structure. In most cases the behavior of the mutant lysozymes can be easily rationalized, but there are a few instances where this is not so.

Original language	English (US)
Pages (from-to)	81-98
Number of pages	18
Journal	Journal of Molecular Biology
Volume	118
Issue number	1
DOIs	https://doi.org/10.1016/0022-2836(78)90245-0
State	Published - Jan 5 1978

Funding

Mutant strains eJR14eJD5 and c,J28eJD8 wcrc kintlly provicktl by Drs A. Ts\lpt’itt ant1 M. B&la, rind cseHU58 by Dr D.-H. Liebscher. Tllis study was supported in part by grants from the National Institutes of Health (GM21967, GM19795, GM20066), the National Scienw Foundation (PCM75-18407), and a Public Health Service Career Development AwarcI to one of us (B.W.M.) (GM 70585) from tjlw Institutes of General Medical Sciancc-.

ASJC Scopus subject areas

Structural Biology
Molecular Biology

Access to Document

10.1016/0022-2836(78)90245-0

Cite this

@article{920e1006791748018f34fa9b06297603,

title = "Structure of the lysozyme from bacteriophage T4: An electron density map at 2.4 {\AA} resolution",

abstract = "The three-dimensional structure of the lysozyme from bacteriophage T4 has been determined from a 2.4 {\AA} resolution electron density map. The map, determined using isomorphous replacement and anomalous scattering differences from platinum and mercury-substituted crystals, reveals the approximate conformation of the molecule unambiguously. Details of the structure determination are presented and the conformation of the molecule is described in detail. The observed properties of those mutant lysozymes for which the changes in the amino acid sequence have been determined are discussed in the light of the three-dimensional structure. In most cases the behavior of the mutant lysozymes can be easily rationalized, but there are a few instances where this is not so.",

author = "Remington, {S. J.} and Anderson, {W. F.} and J. Owen and Eyck, {L. F Ten} and Grainger, {C. T.} and Matthews, {B. W.}",

note = "Funding Information: Mutant strains eJR14eJD5 and c,J28eJD8 wcrc kintlly provicktl by Drs A. Ts\lpt{\textquoteright}itt ant1 M. B&la, rind cseHU58 by Dr D.-H. Liebscher. Tllis study was supported in part by grants from the National Institutes of Health (GM21967, GM19795, GM20066), the National Scienw Foundation (PCM75-18407), and a Public Health Service Career Development AwarcI to one of us (B.W.M.) (GM 70585) from tjlw Institutes of General Medical Sciancc-.",

year = "1978",

month = jan,

day = "5",

doi = "10.1016/0022-2836(78)90245-0",

language = "English (US)",

volume = "118",

pages = "81--98",

journal = "Journal of Molecular Biology",

issn = "0022-2836",

publisher = "Academic Press",

number = "1",

}

TY - JOUR

T1 - Structure of the lysozyme from bacteriophage T4

T2 - An electron density map at 2.4 Å resolution

AU - Remington, S. J.

AU - Anderson, W. F.

AU - Owen, J.

AU - Eyck, L. F Ten

AU - Grainger, C. T.

AU - Matthews, B. W.

N1 - Funding Information: Mutant strains eJR14eJD5 and c,J28eJD8 wcrc kintlly provicktl by Drs A. Ts\lpt’itt ant1 M. B&la, rind cseHU58 by Dr D.-H. Liebscher. Tllis study was supported in part by grants from the National Institutes of Health (GM21967, GM19795, GM20066), the National Scienw Foundation (PCM75-18407), and a Public Health Service Career Development AwarcI to one of us (B.W.M.) (GM 70585) from tjlw Institutes of General Medical Sciancc-.

PY - 1978/1/5

Y1 - 1978/1/5

N2 - The three-dimensional structure of the lysozyme from bacteriophage T4 has been determined from a 2.4 Å resolution electron density map. The map, determined using isomorphous replacement and anomalous scattering differences from platinum and mercury-substituted crystals, reveals the approximate conformation of the molecule unambiguously. Details of the structure determination are presented and the conformation of the molecule is described in detail. The observed properties of those mutant lysozymes for which the changes in the amino acid sequence have been determined are discussed in the light of the three-dimensional structure. In most cases the behavior of the mutant lysozymes can be easily rationalized, but there are a few instances where this is not so.

AB - The three-dimensional structure of the lysozyme from bacteriophage T4 has been determined from a 2.4 Å resolution electron density map. The map, determined using isomorphous replacement and anomalous scattering differences from platinum and mercury-substituted crystals, reveals the approximate conformation of the molecule unambiguously. Details of the structure determination are presented and the conformation of the molecule is described in detail. The observed properties of those mutant lysozymes for which the changes in the amino acid sequence have been determined are discussed in the light of the three-dimensional structure. In most cases the behavior of the mutant lysozymes can be easily rationalized, but there are a few instances where this is not so.

UR - http://www.scopus.com/inward/record.url?scp=0017821233&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017821233&partnerID=8YFLogxK

U2 - 10.1016/0022-2836(78)90245-0

DO - 10.1016/0022-2836(78)90245-0

M3 - Article

C2 - 625058

AN - SCOPUS:0017821233

SN - 0022-2836

VL - 118

SP - 81

EP - 98

JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

IS - 1

ER -

Structure of the lysozyme from bacteriophage T4: An electron density map at 2.4 Å resolution

Abstract

Funding

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this