Structure of the Newcastle disease virus F protein in the post-fusion conformation

Kurt Swanson, Xiaolin Wen, George P. Leser, Reay G. Paterson, Robert A. Lamb, Theodore S. Jardetzky*

*Corresponding author for this work

Research output: Contribution to journalArticle

66 Scopus citations

Abstract

The paramyxovirus F protein is a class I viral membrane fusion protein which undergoes a significant refolding transition during virus entry. Previous studies of the Newcastle disease virus, human parainfluenza virus 3 and parainfluenza virus 5 F proteins revealed differences in the pre- and post-fusion structures. The NDV Queensland (Q) F structure lacked structural elements observed in the other two structures, which are key to the refolding and fusogenic activity of F. Here we present the NDV Australia-Victoria (AV) F protein post-fusion structure and provide EM evidence for its folding to a pre-fusion form. The NDV AV F structure contains heptad repeat elements missing in the previous NDV Q F structure, forming a post-fusion six-helix bundle (6HB) similar to the post-fusion hPIV3 F structure. Electrostatic and temperature factor analysis of the F structures points to regions of these proteins that may be functionally important in their membrane fusion activity.

Original languageEnglish (US)
Pages (from-to)372-379
Number of pages8
JournalVirology
Volume402
Issue number2
DOIs
StatePublished - Jul 2010

Keywords

  • Class I viral fusion proteins
  • Membrane fusion
  • NDV
  • Paramyxovirus
  • Virus entry

ASJC Scopus subject areas

  • Virology

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