Structure of the uncleaved ectodomain of the paramyxovirus (hPIV3) fusion protein

Hsien Sheng Yin, Reay G. Paterson, Xiaolin Wen, Robert A. Lamb, Theodore S. Jardetzky*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

250 Scopus citations

Abstract

Class I viral fusion proteins share common mechanistic and structural features but little sequence similarity. Structural insights into the protein conformational changes associated with membrane fusion are based largely on studies of the influenza virus hemagglutinin in pre- and postfusion conformations. Here, we present the crystal structure of the secreted, uncleaved ectodomain of the paramyxovirus, human parainfluenza virus 3 fusion (F) protein, a member of the class I viral fusion protein group. The secreted human parainfluenza virus 3 F forms a trimer with distinct head, neck, and stalk regions. Unexpectedly, the structure reveals a six-helix bundle associated with the postfusion form of F, suggesting that the anchor-minus ectodomain adopts a conformation largely similar to the postfusion state. The transmembrane anchor domains of F may therefore profoundly influence the folding energetics that establish and maintain a metastable, prefusion state.

Original languageEnglish (US)
Pages (from-to)9288-9293
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume102
Issue number26
DOIs
StatePublished - Jun 28 2005

Keywords

  • Atomic structure
  • Class I fusion protein
  • Membrane fusion

ASJC Scopus subject areas

  • General

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