Structures of two intermediate filament-binding fragments of desmoplakin reveal a unique repeat motif structure

Hee Jung Choi, Shaun Park-Snyder, Lauren T. Pascoe, Kathleen Janee Green, William I. Weis

Research output: Contribution to journalArticlepeer-review

88 Scopus citations

Abstract

Desmosomes are intercellular junctions in which cadherin cell adhesion molecules are linked to the intermediate filament (IF) system. Desmoplakin is a member of the plakin family of IF-binding proteins. The C-terminal domain of desmoplakin (DPCT) mediates binding to IFs in desmosomes. The DPCT sequence contains three regions, termed A, B and C, consisting of 4.5 copies of a 38-amino acid repeat motif. We demonstrate that these regions form discrete subdomains that bind to IFs and report the crystal structures of domains B and C. In contrast to the elongated structures formed by other kinds of repeat motifs, the plakin repeats form a globular structure with a unique fold. A conserved basic groove found on the domain may represent an IF-binding site.

Original languageEnglish (US)
Pages (from-to)612-620
Number of pages9
JournalNature Structural Biology
Volume9
Issue number8
DOIs
StatePublished - Jan 1 2002

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Genetics

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