Studies of a chemically modified oligodeoxynucleotide containing a 5-atom amide backbone which exhibits improved binding to RNA

C. J. Wilds, G. Minasov, F. Natt, P. Von Matt, K. H. Altmann, M. Egli*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

Chimeric oligodeoxyribonucleotides where the phosphodiester linkage -C3′O-PO2- -O-CH2-C4′- of DNA is substituted by the amide linkage -C3′-CH2CH*(CH3) -CO-NH-CH2-C4′ (*either R or S stereochemistry) have been prepared and their binding to RNA targets have been investigated. Incorporation of a single amide unit increases the Tm by approximately 1.4-1.9°C. Circular dichroic spectra of these modified duplexes are similar to the wildtype DNA/RNA.

Original languageEnglish (US)
Pages (from-to)991-994
Number of pages4
JournalNucleosides, Nucleotides and Nucleic Acids
Volume20
Issue number4-7
DOIs
StatePublished - Sep 17 2001

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Genetics

Fingerprint Dive into the research topics of 'Studies of a chemically modified oligodeoxynucleotide containing a 5-atom amide backbone which exhibits improved binding to RNA'. Together they form a unique fingerprint.

Cite this