Chimeric oligodeoxyribonucleotides where the phosphodiester linkage -C3′O-PO2- -O-CH2-C4′- of DNA is substituted by the amide linkage -C3′-CH2CH*(CH3) -CO-NH-CH2-C4′ (*either R or S stereochemistry) have been prepared and their binding to RNA targets have been investigated. Incorporation of a single amide unit increases the Tm by approximately 1.4-1.9°C. Circular dichroic spectra of these modified duplexes are similar to the wildtype DNA/RNA.
ASJC Scopus subject areas
- Molecular Medicine