Studies on the crystallization of the cro protein-pseudo OR3 complex

Richard G. Brennan; Jim Kim; Yoshinori Takeda; Wayne F. Anderson; Brian W. Matthews

doi:10.1016/0022-0248(86)90189-2

Studies on the crystallization of the cro protein-pseudo O_R3 complex

Richard G. Brennan^*, Jim Kim, Yoshinori Takeda, Wayne F. Anderson, Brian W. Matthews

^*Corresponding author for this work

Biochemistry and Molecular Genetics

Research output: Contribution to journal › Article › peer-review

Abstract

Crystals of a complex of cro protein and a 17 base-pair DNA operator fragment have been grown by vapor diffusion and controlled evaporation methods. The former method, although successful, was accompanied by crystal clustering, excessive nucleation, and a sticky crystalline precipitate. The latter technique eliminated these crystallization flaws and resulted in large crystals suitable for diffraction studies. Details of the crystallization procedures are presented.

Original language	English (US)
Pages (from-to)	715-718
Number of pages	4
Journal	Journal of Crystal Growth
Volume	76
Issue number	3
DOIs	https://doi.org/10.1016/0022-0248(86)90189-2
State	Published - Aug 2 1986

Funding

This work was supported by grants from the National Institutes of Health (GM 20066) to B.W.M. and (GM 30894) to Y.T., The National Science Foundation (PCM 8312151) to B.W.M. and by an NIH postdoctoral fellowship (GM 10476) to R.G.B.

ASJC Scopus subject areas

Condensed Matter Physics
Inorganic Chemistry
Materials Chemistry

Access to Document

10.1016/0022-0248(86)90189-2

Cite this

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title = "Studies on the crystallization of the cro protein-pseudo OR3 complex",

abstract = "Crystals of a complex of cro protein and a 17 base-pair DNA operator fragment have been grown by vapor diffusion and controlled evaporation methods. The former method, although successful, was accompanied by crystal clustering, excessive nucleation, and a sticky crystalline precipitate. The latter technique eliminated these crystallization flaws and resulted in large crystals suitable for diffraction studies. Details of the crystallization procedures are presented.",

author = "Brennan, {Richard G.} and Jim Kim and Yoshinori Takeda and Anderson, {Wayne F.} and Matthews, {Brian W.}",

note = "Funding Information: This work was supported by grants from the National Institutes of Health (GM 20066) to B.W.M. and (GM 30894) to Y.T., The National Science Foundation (PCM 8312151) to B.W.M. and by an NIH postdoctoral fellowship (GM 10476) to R.G.B.",

year = "1986",

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doi = "10.1016/0022-0248(86)90189-2",

language = "English (US)",

volume = "76",

pages = "715--718",

journal = "Journal of Crystal Growth",

issn = "0022-0248",

publisher = "Elsevier B.V.",

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T1 - Studies on the crystallization of the cro protein-pseudo OR3 complex

AU - Brennan, Richard G.

AU - Kim, Jim

AU - Takeda, Yoshinori

AU - Anderson, Wayne F.

AU - Matthews, Brian W.

N1 - Funding Information: This work was supported by grants from the National Institutes of Health (GM 20066) to B.W.M. and (GM 30894) to Y.T., The National Science Foundation (PCM 8312151) to B.W.M. and by an NIH postdoctoral fellowship (GM 10476) to R.G.B.

PY - 1986/8/2

Y1 - 1986/8/2

N2 - Crystals of a complex of cro protein and a 17 base-pair DNA operator fragment have been grown by vapor diffusion and controlled evaporation methods. The former method, although successful, was accompanied by crystal clustering, excessive nucleation, and a sticky crystalline precipitate. The latter technique eliminated these crystallization flaws and resulted in large crystals suitable for diffraction studies. Details of the crystallization procedures are presented.

AB - Crystals of a complex of cro protein and a 17 base-pair DNA operator fragment have been grown by vapor diffusion and controlled evaporation methods. The former method, although successful, was accompanied by crystal clustering, excessive nucleation, and a sticky crystalline precipitate. The latter technique eliminated these crystallization flaws and resulted in large crystals suitable for diffraction studies. Details of the crystallization procedures are presented.

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