Studies on the crystallization of the cro protein-pseudo OR3 complex

Richard G. Brennan*, Jim Kim, Yoshinori Takeda, Wayne F. Anderson, Brian W. Matthews

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Crystals of a complex of cro protein and a 17 base-pair DNA operator fragment have been grown by vapor diffusion and controlled evaporation methods. The former method, although successful, was accompanied by crystal clustering, excessive nucleation, and a sticky crystalline precipitate. The latter technique eliminated these crystallization flaws and resulted in large crystals suitable for diffraction studies. Details of the crystallization procedures are presented.

Original languageEnglish (US)
Pages (from-to)715-718
Number of pages4
JournalJournal of Crystal Growth
Volume76
Issue number3
DOIs
StatePublished - Aug 2 1986

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Inorganic Chemistry
  • Materials Chemistry

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