Issledovanie belkovogo kompleksa iz mozga, ponizhaiushchego viazkost' aktina. I. Sostav i nekotorye svoǐstva kompleksa.

Translated title of the contribution: Study of a protein complex from the brain which reduces actin viscosity. I. Composition and various properties of the complex

A. B. Verkhovskiǐ*, I. G. Surgucheva, V. I. Gel'fand

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The method of isolation from bovine brain of a preparation containing 90 kDa- and 42 kDa-proteins is described. This preparation shortens actin filaments and therefore decreases viscosity of F-actin. The 42 kDa-component was identified as actin by one-dimensional peptide mapping. Quantitative densitometry has demonstrated that 90 kDa-protein and actin are present in the preparation in equimolar ratio. Fractionation of the preparation by gel-filtration, analytical centrifugation or electrophoresis under non-denaturing conditions showed that 90 kDa-protein and actin are in a light complex. This complex consists of one actin molecule and one molecule of 90 kDa-protein and has a sedimentation coefficient of 3.5S. Both beta- and gamma-isoelectric forms of actin are present in the complex.

Translated title of the contributionStudy of a protein complex from the brain which reduces actin viscosity. I. Composition and various properties of the complex
Original languageRussian
Pages (from-to)922-928
Number of pages7
JournalMolekuliarnaia biologiia
Volume20
Issue number4
StatePublished - Jul 1 1986

ASJC Scopus subject areas

  • Medicine(all)

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