In order to prevent the loss of microbes, added anaerobic ammonia oxidation to (ASBR) reactor plus membrane as the growth of anaerobic ammonia oxidation bacteria carrier. After 30d cultivate, the activity of anammox bacteria restore to the original level, and improve TN volume load train anammox bacteria. The whole process of NH4 +-N and NO2 --N removal rate has remained at more than 90%.After the activity recovery of anammox NH4 +-N, NO2 --N the ratio of consumption to the NO3 --N generation finally tends to a certain value (1. 00b 1. 30 b 0. 25).In the process of cultivate, the sludge was gradually changed from black to red brown color.Results show that the ASBR is well suited for anammox enrichment culture of bacteria.