Studying DNA translocation in nanocapillaries using single molecule fluorescence

Vivek V. Thacker, Sandip Ghosal, Silvia Hernández-Ainsa, Nicholas A.W. Bell, Ulrich F. Keyser*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

We demonstrate simultaneous measurements of DNA translocation into glass nanopores using ionic current detection and fluorescent imaging. We verify the correspondence between the passage of a single DNA molecule through the nanopore and the accompanying characteristic ionic current blockage. By tracking the motion of individual DNA molecules in the nanocapillary perpendicular to the optical axis and using a model, we can extract an effective mobility constant for DNA in our geometry under high electric fields.

Original languageEnglish (US)
Article number223704
JournalApplied Physics Letters
Volume101
Issue number22
DOIs
StatePublished - Nov 26 2012

ASJC Scopus subject areas

  • Physics and Astronomy (miscellaneous)

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