Studying DNA translocation in nanocapillaries using single molecule fluorescence

Vivek V. Thacker; Sandip Ghosal; Silvia Hernández-Ainsa; Nicholas A.W. Bell; Ulrich F. Keyser

doi:10.1063/1.4768929

Studying DNA translocation in nanocapillaries using single molecule fluorescence

Vivek V. Thacker, Sandip Ghosal, Silvia Hernández-Ainsa, Nicholas A.W. Bell, Ulrich F. Keyser^*

^*Corresponding author for this work

Mechanical Engineering

Research output: Contribution to journal › Article › peer-review

39 Scopus citations

Abstract

We demonstrate simultaneous measurements of DNA translocation into glass nanopores using ionic current detection and fluorescent imaging. We verify the correspondence between the passage of a single DNA molecule through the nanopore and the accompanying characteristic ionic current blockage. By tracking the motion of individual DNA molecules in the nanocapillary perpendicular to the optical axis and using a model, we can extract an effective mobility constant for DNA in our geometry under high electric fields.

Original language	English (US)
Article number	223704
Journal	Applied Physics Letters
Volume	101
Issue number	22
DOIs	https://doi.org/10.1063/1.4768929
State	Published - Nov 26 2012

Funding

We thank S. Pagliara for help with tracking software in labview . V.V.T. gratefully acknowledges funding from the Cambridge Commonwealth Trust, the Jawaharlal Nehru Memorial Trust, and the Emmy Noether program. S.G. acknowledges funding from the NIH (USA) under Grant R01HG004842 and from the Leverhulme Trust (UK). S.H.-A., N.A.W.B., and U.F.K. acknowledge funding from an ERC starting grant, the EPSRC NanoDTC program and the Emmy Noether program, respectively.

ASJC Scopus subject areas

Physics and Astronomy (miscellaneous)

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10.1063/1.4768929

Cite this

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abstract = "We demonstrate simultaneous measurements of DNA translocation into glass nanopores using ionic current detection and fluorescent imaging. We verify the correspondence between the passage of a single DNA molecule through the nanopore and the accompanying characteristic ionic current blockage. By tracking the motion of individual DNA molecules in the nanocapillary perpendicular to the optical axis and using a model, we can extract an effective mobility constant for DNA in our geometry under high electric fields.",

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AU - Keyser, Ulrich F.

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N2 - We demonstrate simultaneous measurements of DNA translocation into glass nanopores using ionic current detection and fluorescent imaging. We verify the correspondence between the passage of a single DNA molecule through the nanopore and the accompanying characteristic ionic current blockage. By tracking the motion of individual DNA molecules in the nanocapillary perpendicular to the optical axis and using a model, we can extract an effective mobility constant for DNA in our geometry under high electric fields.

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Studying DNA translocation in nanocapillaries using single molecule fluorescence

Abstract

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