@article{32cc5442bb764b03a23be4f325656f36,
title = "Sub-10-nm imaging of nucleic acids using spectroscopic intrinsic-contrast photon-localization optical nanoscopy (SICLON)",
abstract = "Elucidating chromatin structure in vitro requires resolution below 10 nm to visualize the mononucleosome has been an ongoing challenge. In this work, we achieve sub-10-nm imaging of nucleic acids via spectroscopic intrinsic-contrast photon-localization optical nanoscopy (SICLON) without the use of external labels. SICLON leverages two key innovations: using endogenous nucleotides as the emission source and a custom-made imaging system that can simultaneously record the position and optical spectra of emitting molecules. With a novel spectral regression algorithm that identifies the spectroscopic fingerprints of neighboring molecules that were previously indistinguishable, we demonstrate the utility of SICLON by visualizing unlabeled poly-nucleotides and linear single-stranded DNA fibers with a resolution of 6.2 nm.",
author = "Adam Eshein and Yue Li and Biqin Dong and Almassalha, {Luay M.} and Chandler, {John E.} and Nguyen, {The Quyen} and Hujsak, {Karl A.} and Dravid, {Vinayak P.} and Cheng Sun and Zhang, {Hao F.} and Vadim Backman",
note = "Funding Information: National Cancer Institute (NCI) (R01CA200064, U54CA193419); National Institute of Biomedical Imaging and Bioengineering (NIBIB) (F31EB022414); National Science Foundation (NSF) (CBET-1240416, CBET-1706642); LUNGevity Foundation; Soft and Hybrid Nanotechnology Experimental (SHyNE) Resource (NSF ECCS-1542205); MRSEC program (NSF DMR-1720139); International Institute for Nanotechnology (IIN); Keck Foundation; State of Illinois, through the IIN, and the Air Force Office of Scientific Research (AFOSR) (FA9550-17-1-0348). This work made use of the SPID facility of Northwestern University{\textquoteright}s NUANCE Center, which has received support from the SHyNE; MRSEC at the Materials Research Center; IIN; Keck Foundation; and the State of Illinois, through the IIN, and the Air Force Office of Scientific Research. Funding Information: Funding. National Cancer Institute (NCI) (R01CA200064, U54CA193419); National Institute of Biomedical Imaging and Bioengineering (NIBIB) (F31EB022414); National Science Foundation (NSF) (CBET-1240416, CBET-1706642); LUNGevity Foundation; Soft and Hybrid Nanotechnology Experimental (SHyNE) Resource (NSF ECCS-1542205); MRSEC program (NSF DMR-1720139); International Institute for Nanotechnology (IIN); Keck Foundation; State of Illinois, through the IIN, and the Air Force Office of Scientific Research (AFOSR) (FA9550-17-1-0348). Publisher Copyright: {\textcopyright} 2018 Optical Society of America.",
year = "2018",
month = dec,
day = "1",
doi = "10.1364/OL.43.005817",
language = "English (US)",
volume = "43",
pages = "5817--5820",
journal = "Optics Letters",
issn = "0146-9592",
publisher = "The Optical Society",
number = "23",
}