Sub-micron patterning on polymer films for protein arrays

Karen L. Christman; Michael V. Requa; Vanessa D. Enriquez-Rios; Paula Mendes; J. Fraser Stoddart; Kimberly L. Turner; Heather D. Maynard

doi:10.1557/proc-0900-o09-35

Sub-micron patterning on polymer films for protein arrays

Karen L. Christman, Michael V. Requa, Vanessa D. Enriquez-Rios, Paula Mendes, J. Fraser Stoddart, Kimberly L. Turner, Heather D. Maynard^*

^*Corresponding author for this work

Chemistry

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Patterning proteins at the sub-micron and nanoscale has many uses, including fabrication of protein arrays for diagnostic and sensor applications. Many groups have reported micron scale protein patterns using photolithography; however, smaller scales have not been realized. In this study, we demonstrate sub-micron protein patterns using photolithography. Patterning is achieved by chemical transformation of pH-reactive polymer films. Site-specific immobilization of streptavidin within a protein resistant background is demonstrated. This methodology could be utilized for the development of high density proteins arrays for biotechnology applications.

Original language	English (US)
Title of host publication	Nanoparticles and Nanostructures in Sensors and Catalysis
Publisher	Materials Research Society
Pages	329-333
Number of pages	5
ISBN (Print)	1558998543, 9781558998544
DOIs	https://doi.org/10.1557/proc-0900-o09-35
State	Published - 2005
Event	2005 MRS Fall Meeting - Boston, MA, United States Duration: Nov 28 2005 → Dec 2 2005

Publication series

Name	Materials Research Society Symposium Proceedings
Volume	900
ISSN (Print)	0272-9172

Other

Other	2005 MRS Fall Meeting
Country/Territory	United States
City	Boston, MA
Period	11/28/05 → 12/2/05

ASJC Scopus subject areas

General Materials Science
Condensed Matter Physics
Mechanics of Materials
Mechanical Engineering

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10.1557/proc-0900-o09-35

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Christman, K. L., Requa, M. V., Enriquez-Rios, V. D., Mendes, P., Fraser Stoddart, J., Turner, K. L., & Maynard, H. D. (2005). Sub-micron patterning on polymer films for protein arrays. In Nanoparticles and Nanostructures in Sensors and Catalysis (pp. 329-333). (Materials Research Society Symposium Proceedings; Vol. 900). Materials Research Society. https://doi.org/10.1557/proc-0900-o09-35

@inproceedings{0f44c2cf74474894a9f8ee63629b3da4,

title = "Sub-micron patterning on polymer films for protein arrays",

abstract = "Patterning proteins at the sub-micron and nanoscale has many uses, including fabrication of protein arrays for diagnostic and sensor applications. Many groups have reported micron scale protein patterns using photolithography; however, smaller scales have not been realized. In this study, we demonstrate sub-micron protein patterns using photolithography. Patterning is achieved by chemical transformation of pH-reactive polymer films. Site-specific immobilization of streptavidin within a protein resistant background is demonstrated. This methodology could be utilized for the development of high density proteins arrays for biotechnology applications.",

author = "Christman, {Karen L.} and Requa, {Michael V.} and Enriquez-Rios, {Vanessa D.} and Paula Mendes and {Fraser Stoddart}, J. and Turner, {Kimberly L.} and Maynard, {Heather D.}",

year = "2005",

doi = "10.1557/proc-0900-o09-35",

language = "English (US)",

isbn = "1558998543",

series = "Materials Research Society Symposium Proceedings",

publisher = "Materials Research Society",

pages = "329--333",

booktitle = "Nanoparticles and Nanostructures in Sensors and Catalysis",

note = "2005 MRS Fall Meeting ; Conference date: 28-11-2005 Through 02-12-2005",

}

Christman, KL, Requa, MV, Enriquez-Rios, VD, Mendes, P, Fraser Stoddart, J, Turner, KL & Maynard, HD 2005, Sub-micron patterning on polymer films for protein arrays. in Nanoparticles and Nanostructures in Sensors and Catalysis. Materials Research Society Symposium Proceedings, vol. 900, Materials Research Society, pp. 329-333, 2005 MRS Fall Meeting, Boston, MA, United States, 11/28/05. https://doi.org/10.1557/proc-0900-o09-35

Sub-micron patterning on polymer films for protein arrays. / Christman, Karen L.; Requa, Michael V.; Enriquez-Rios, Vanessa D. et al.
Nanoparticles and Nanostructures in Sensors and Catalysis. Materials Research Society, 2005. p. 329-333 (Materials Research Society Symposium Proceedings; Vol. 900).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Sub-micron patterning on polymer films for protein arrays

AU - Christman, Karen L.

AU - Requa, Michael V.

AU - Enriquez-Rios, Vanessa D.

AU - Mendes, Paula

AU - Fraser Stoddart, J.

AU - Turner, Kimberly L.

AU - Maynard, Heather D.

PY - 2005

Y1 - 2005

N2 - Patterning proteins at the sub-micron and nanoscale has many uses, including fabrication of protein arrays for diagnostic and sensor applications. Many groups have reported micron scale protein patterns using photolithography; however, smaller scales have not been realized. In this study, we demonstrate sub-micron protein patterns using photolithography. Patterning is achieved by chemical transformation of pH-reactive polymer films. Site-specific immobilization of streptavidin within a protein resistant background is demonstrated. This methodology could be utilized for the development of high density proteins arrays for biotechnology applications.

AB - Patterning proteins at the sub-micron and nanoscale has many uses, including fabrication of protein arrays for diagnostic and sensor applications. Many groups have reported micron scale protein patterns using photolithography; however, smaller scales have not been realized. In this study, we demonstrate sub-micron protein patterns using photolithography. Patterning is achieved by chemical transformation of pH-reactive polymer films. Site-specific immobilization of streptavidin within a protein resistant background is demonstrated. This methodology could be utilized for the development of high density proteins arrays for biotechnology applications.

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DO - 10.1557/proc-0900-o09-35

M3 - Conference contribution

AN - SCOPUS:34249952337

SN - 1558998543

SN - 9781558998544

T3 - Materials Research Society Symposium Proceedings

SP - 329

EP - 333

BT - Nanoparticles and Nanostructures in Sensors and Catalysis

PB - Materials Research Society

T2 - 2005 MRS Fall Meeting

Y2 - 28 November 2005 through 2 December 2005

ER -

Sub-micron patterning on polymer films for protein arrays

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ASJC Scopus subject areas

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