Subacute infection with temperature-sensitive vesicular stomatitis virus mutant g41 in the central nervous system of mice. I. clinical and virologic studies

Stanley G. Rabinowitz, Terry C. Johnson*, Mauro Carlo Dal Canto

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Inoculation of three- to four-week-old BALB/c mice with temperature-sensitive (ts) vesicular stomatitis virus mutant G41 produced a subacute neurological disease, initially characterized by development of lethargy, hunched posture, and ruffled fur within five to seven days after infection. More than 90% of infected mice developed these clinical signs. In -60% of infected mice, the initial neurological signs proceeded to striking hind-limb paralysis and weight loss. These signs usually appeared by seven to nine days after infection and lasted for 21-28 days. Only 16% of the mice died as a result of infection; death usually occurred eight to 12 days after infection. Most of the infected mice recovered from the acute phase of disease and appeared normal by four weeks after infection. However, hind-limb paralysis persisted in 4% of the mice for as long as the mice were observed, i.e., 42 days. The mutant ti-G41 was recovered from the brains and spinal cords of infected mice for the first seven days after infection. Peak titers of virus were modest, 104-105 pfu/ml in brain tissue and 103-104 pfu/ml in spinal cord tissue. Virus isolated after in vivo infection was temperature-sensitive and thus not revertant wild-type virus. Although virus was recoverable by homogenization for only the first seven days of infection, use of cocultivation techniques permitted the detection of ts-G41 in brains and spinal cords of infected animals for as long as 21 days after infection. Virus recovered by cocultivation was also temperature-sensitive.

Original languageEnglish (US)
Pages (from-to)26-35
Number of pages10
JournalJournal of Infectious Diseases
Volume139
Issue number1
DOIs
StatePublished - Jan 1 1979

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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