TY - JOUR
T1 - Subcellular distribution of two enzyme systems which degrade 3′-phosphoadenosine 5′-phosphosulfate ("Active sulfate")
AU - Austin, James
AU - Armstrong, Donald
AU - Stumpf, David
AU - Luttenegger, Thomas
AU - Dragoo, Mildred
N1 - Funding Information:
These studies were supported by U.S. Public Health Service grant No. NB-o77oi. Professor B. K. Bachhawat generously supplied E~sS)PAPS.
PY - 1969/10/7
Y1 - 1969/10/7
N2 - 1. 1. The subcellular distribution of two enzyme systems which degrade 3′-phosphoadenosine 5′-phosphosulfate (PAPS) ("active sulfate") has been determined. PAPS sulfatase has the wider distribution and accounts for most of the PAPS hydrolyzed. The 3′-phosphoadenosine 5′-phosphate (PAP) thus formed might be available to serve as the requisite coenzyme in phenolic sulfotransferase reactions (detoxification). 2. 2. PAPS-phosphatase activity has a more restricted distribution. It is concentrated in the lighter vesicles of the smooth endoplasmic reticulum (Smooth-4 fraction). It is chiefly present in the soluble portion of this fraction. The adenosine 5′-phosphosulfate (APS) thus formed might be available for conversion back to PAPS as required by the needs of the sulfotransferase systems (lipid, mucopolysaccharide, steroid) also localized in the smooth reticulum.
AB - 1. 1. The subcellular distribution of two enzyme systems which degrade 3′-phosphoadenosine 5′-phosphosulfate (PAPS) ("active sulfate") has been determined. PAPS sulfatase has the wider distribution and accounts for most of the PAPS hydrolyzed. The 3′-phosphoadenosine 5′-phosphate (PAP) thus formed might be available to serve as the requisite coenzyme in phenolic sulfotransferase reactions (detoxification). 2. 2. PAPS-phosphatase activity has a more restricted distribution. It is concentrated in the lighter vesicles of the smooth endoplasmic reticulum (Smooth-4 fraction). It is chiefly present in the soluble portion of this fraction. The adenosine 5′-phosphosulfate (APS) thus formed might be available for conversion back to PAPS as required by the needs of the sulfotransferase systems (lipid, mucopolysaccharide, steroid) also localized in the smooth reticulum.
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U2 - 10.1016/0304-4165(69)90006-3
DO - 10.1016/0304-4165(69)90006-3
M3 - Article
C2 - 4310534
AN - SCOPUS:0014667290
VL - 192
SP - 29
EP - 36
JO - Biochimica et Biophysica Acta - General Subjects
JF - Biochimica et Biophysica Acta - General Subjects
SN - 0304-4165
IS - 1
ER -