Subcellular Fractionation Suitable for Studies of RNA and Protein Trafficking

Biljana Culjkovic-Kraljacic*, Katherine L.B. Borden

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

The nuclear pore complex is the major conduit for trafficking between the nucleus and cytoplasm. Nuclear import and export of both proteins and RNAs represent important functional steps for many biological processes. One of the major means to study NPC activity and the nuclear and cytoplasmic distribution of proteins and RNAs is through biochemical fractionation. Here, we describe detailed methods to generate high quality nuclear and cytoplasmic fractions simultaneously capturing RNA and proteins which can be used subsequently for a wide array of biochemical characterizations including proteomics and next generation sequencings.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages91-104
Number of pages14
DOIs
StatePublished - 2022

Publication series

NameMethods in Molecular Biology
Volume2502
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Localization
  • Nuclear- Cytoplasmic Fractionation
  • Protein
  • RNA

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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