Subconductance-state currents generated by imidacloprid at the nicotinic acetylcholine receptor in PC 12 cells

Keiichi Nagata, Gary L. Aistrup, Jin Ho Song, Toshio Narahashi*

*Corresponding author for this work

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

Nitromethylene heterocyclic insecticides are known to act on the nicotinic acetylcholine receptor-channel complex. The effects of the nitromethylene heterocycle imidacloprid on the nicotinic acetylcholine receptor of clonal rat pheochromocytoma (PC 12) cells were studied using the single-channel patch clamp technique. Imidacloprid generated single-channel currents of multiple conductance states in PC12 cells. Both acetylcholine and imidacloprid induced single-channel currents of main conductance and subconductance states with conductances of 33.3 and 9.4 pS by acetylcholine and 30.4 and 9.8 pS by imidacloprid. However, the main conductance currents were generated predominantly by acetylcholine, whereas the subconductance currents were generated predominantly by imidacloprid. Partial block of acetylcholine-induced currents by imidacloprid may be explained on the basis of the single-channel behavior.

Original languageEnglish (US)
Pages (from-to)1025-1028
Number of pages4
JournalNeuroreport
Volume7
Issue number5
DOIs
StatePublished - 1996

Keywords

  • Imidacloprid
  • Insecticide
  • Ion channel
  • Nicotinic acetylcholine receptor
  • Nitromethylene heterocycle
  • PC12 cell
  • Subconductance state

ASJC Scopus subject areas

  • Neuroscience(all)

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