Submerged culture production of chitinase by Trichoderma harzianum in stirred tank bioreactors - The influence of agitator speed

P. Arthur Felse, T. Panda*

*Corresponding author for this work

Research output: Contribution to journalArticle

40 Scopus citations

Abstract

The chitinase fermentation process utilizing chitin as the sole carbon source was investigated in a stirred tank bioreactor. Agitator speed of 224 rpm was found to be most suitable for cell growth as well as for chitinase production. Chitinase yield decreased rapidly at higher agitator speed, while decrease in cell yield at higher agitator speed was not rapid. Probably, mass transfer limitation was predominant in the fermentation process at lower agitator speed. Higher agitator speed appears to reduce chitinase production.

Original languageEnglish (US)
Pages (from-to)115-120
Number of pages6
JournalBiochemical Engineering Journal
Volume4
Issue number2
DOIs
StatePublished - Jan 1 2000

Keywords

  • Agitation
  • Bioreactors
  • Chitinase
  • Kinetics
  • Trichoderma harzianum
  • Yield

ASJC Scopus subject areas

  • Biotechnology
  • Environmental Engineering
  • Bioengineering
  • Biomedical Engineering

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