Subnormal albumin gene expression is associated with weight loss in immunodeficient/dna-repair-impaired wasted mice

Claudia R. Libertin*, Paul Weaver, S. Mobarhan, Gayle E Woloschak

*Corresponding author for this work

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Objective: Mice bearing the autosomal recessive mutation wst express a disease syndrome of immunodeficiency, neurologic dysfunction, increased sensitivity to the killing effects of ionizing radiation, and dramatic weight loss that begins at 21 days of age and progresses until death at 28-32 days of age. Because of the reported association between abnormal liver status and weight loss, we designed experiments to examine expression of a variety of liver-specific genes in wst/wst mice relative to littermates (wst/-) and parental strain (BCF,) controls.Method: Animals were individually weighed from ages 21-28 days to determine relative weight comparisons between wst/wst mice and controls. Dot blot hybridizations were set up to quantitate the accumulation of transcripts specific for a-fetoprotein, albumin and other liver-specific gene products.Results: These results showed a 67% reduction in albumin mRNA expression in livers derived from wst/wst mice relative to both controls. Expression of a-fetoprotein, as well as a variety of other liver- specific genes [secretory component (SC), metallothionein (MT-2), cytochrome Pi-450 (Cyt Pi-450), transferrin receptor (Tf Rec), tumor necrosis factor (TNF), and immune-associated antigen (la)], was unaffected.Conclusions: These results suggest a relationship between low albumin expression and wasting syndromes in mice. In addition, our data suggest that the wasted mouse may serve as a unique model for subnormal albumin expression.

Original languageEnglish (US)
Pages (from-to)149-153
Number of pages5
JournalJournal of the American College of Nutrition
Volume13
Issue number2
DOIs
StatePublished - Apr 1 1994

Keywords

  • Albumin deficiency
  • Ataxia telangiectasia
  • Gene expression
  • Wasted mice
  • Wasting syndromes

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

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