Subventricular zone cells remain stable in vitro after brain injury

Maria Luz V Dizon, L. Shin, N. L. Sundholm-Peters, E. Kang, F. G. Szele*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

20 Scopus citations


Subventricular zone (SVZ) cells emigrate toward brain injury but relatively few survive. Thus, if they are to be used for repair, ex vivo expansion and autologous transplantation of SVZ cells may be necessary. Since it is unclear how brain injury alters SVZ cell culture, we studied neurosphere formation, differentiation, and migration, after cortical lesions. The number of neurosphere forming cells from lesioned mice was comparable to controls. Also, the proportion of astrocytes and neurons generated in vitro remained unchanged after cortical lesions. Cell emigration from neurospheres was characterized by increased cell-cell contact after injury in adults and neonates. However, neither molecules implicated in SVZ migration nor the extent of migration changed after injury. Thus, neurospheres can be successfully cultured after extensive brain damage, and they are remarkably stable in vitro, suggesting suitability for ex vivo expansion and autologous transplantation.

Original languageEnglish (US)
Pages (from-to)717-725
Number of pages9
Issue number3
StatePublished - Oct 27 2006


  • fate
  • migration
  • mouse
  • neurogenesis
  • subependymal zone
  • traumatic brain injury

ASJC Scopus subject areas

  • Neuroscience(all)


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