Successful stabilization of graphene oxide in electrolyte solutions: Enhancement of biofunctionalization and cellular uptake

Bong Jin Hong, Owen C. Compton, Zhi An, Ibrahim Eryazici, Sonbinh T. Nguyen*

*Corresponding author for this work

Research output: Contribution to journalArticle

173 Scopus citations

Abstract

Figure Persented: Aqueous dispersions of graphene oxide are inherently unstable in the presence of electrolytes, which screen the electrostatic surface charge on these nanosheets and induce irreversible aggregation. Two complementary strategies, utilizing either electrostatic or steric stabilization, have been developed to enhance the stability of graphene oxide in electrolyte solutions, allowing it to stay dispersed in cell culture media and serum. The electrostatic stabilization approach entails further oxidation of graphene oxide to low C/O ratio (∼1.1) and increases ionic tolerance of these nanosheets. The steric stabilization technique employs an amphiphilic block copolymer that serves as a noncovalently bound surfactant to minimize the aggregate-inducing nanosheet-nanosheet interactions. Both strategies can stabilize graphene oxide nanosheets with large dimensions (>300 nm) in biological media, allowing for an enhancement of >250% in the bioconjugation efficiency of streptavidin in comparison to untreated nanosheets. Notably, both strategies allow the stabilized nanosheets to be readily taken up by cells, demonstrating their excellent performance as potential drug-delivery vehicles.

Original languageEnglish (US)
Pages (from-to)63-73
Number of pages11
JournalACS nano
Volume6
Issue number1
DOIs
StatePublished - Jan 24 2012

Keywords

  • Pluronics
  • biofunctionalization
  • cellular uptake
  • delivery vehicle
  • electrolytic stabilization
  • graphene oxide
  • ionic stabilization

ASJC Scopus subject areas

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)

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