Abstract
Transgenic papaya is widely publicized for controlling papaya ringspot virus. However, the impact of particle bombardment on the genome remains unknown. The transgenic SunUp and its progenitor Sunset genomes were assembled into 351.5 and 350.3 Mb in nine chromosomes, respectively. We identified a 1.64 Mb insertion containing three transgenic insertions in SunUp chromosome 5, consisting of 52 nuclear-plastid, 21 nuclear-mitochondrial and 1 nuclear genomic fragments. A 591.9 kb fragment in chromosome 5 was translocated into the 1.64 Mb insertion. We assembled a gapless 9.8 Mb hermaphrodite-specific region of the Yh chromosome and its 6.0 Mb X counterpart. Resequencing 86 genomes revealed three distinct groups, validating their geographic origin and breeding history. We identified 147 selective sweeps and defined the essential role of zeta-carotene desaturase in carotenoid accumulation during domestication. Our findings elucidated the impact of particle bombardment and improved our understanding of sex chromosomes and domestication to expedite papaya improvement.
Original language | English (US) |
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Pages (from-to) | 715-724 |
Number of pages | 10 |
Journal | Nature Genetics |
Volume | 54 |
Issue number | 5 |
DOIs | |
State | Published - May 2022 |
Funding
This work was supported by US National Science Foundation (NSF) Plant Genome Research Program Award (DBI-1546890 to R.M.), National Natural Science Foundation of China grants (31701889 to J.Y.) and Natural Science Foundation of Fujian Province grants (2018J01601 to J.Y. and 2018J01604 to Xingtan Zhang). The Science and Technology Innovation Fund of Fujian Agriculture and Forestry University (CXZX2020091A to J.Y). This work was supported by US National Science Foundation (NSF) Plant Genome Research Program Award (DBI-1546890 to R.M.), National Natural Science Foundation of China grants (31701889 to J.Y.) and Natural Science Foundation of Fujian Province grants (2018J01601 to J.Y. and 2018J01604 to Xingtan Zhang). The Science and Technology Innovation Fund of Fujian Agriculture and Forestry University (CXZX2020091A to J.Y).
ASJC Scopus subject areas
- Genetics