We describe the preparation of monoiodinated neuropeptide Y (Tyr1-125I-NPY) and monoiodinated peptide YY (Tyr36-125I-PYY). Using these ligands, we detected high, moderate, and low affinity receptor populations in rat brain. Only high and moderate affinity binding sites were suggested by saturation binding studies. Tyr1-125I-NPY bound to 8 ± 3% of the sites with a K(d) of 54 pM (B(max) = 19.4 fmol/mg of protein) and to 92 ± 35 of the sites with a K(d) of 0.92 nM (B(max) = 220.0 fmol/mg of protein). Tyr36-125I-PYY bound to 14 ± 3% of the sites with a K(d) of 23.5 pM (B(max) = 36.4 fmol/mg of protein) and to 86 ± 3% of the sites with a K(d) of 1.9 nM (B(max) = 220.1 fmol/mg of protein). The fragments NPY 13-36 and PYY 13-36 were able to compete with 10 pM Tyr1-125I-NPY for essentially all the binding sites. The fragments were 1 to 2 orders of magnitude less potent than the native peptides. Approximately 50% of the moderate affinity sites, but not the high affinity sites, were reversibly 'lost' in the presence of 5'-guanylyl imidophosphate [Gpp(NH)p], a nonhydrolyzable analog of GTP. Kinetic studies revealed that Tyr1-125I-NPY dissociation could be best described by three dissociation. The proportions of slow and intermediate dissociation matched the proportions of moderate and high affinity binding sites, respectively, as suggested by equilibrium studies. There also existed a phase of fast dissociation. When Gpp(NH)p was added during dissociation, the proportion of slow dissociation decreased to the same extent that the fast dissociation was increased. However, the proportion of intermediate dissociation did not change. We propose that rat brain contains a minor population of high affinity NPY binding sites with an intermediate dissociation rate and no sensitivity to Gpp(NH)p. There is also a major population of moderate affinity binding sites with slow dissociation rate. A component of these sites can convert to a low affinity state with a fast dissociation rate. Gpp(NH)p enhances conversion by stabilizing the low affinity state, thereby producing a 'loss' of moderate affinity binding.
|Original language||English (US)|
|Number of pages||14|
|State||Published - 1988|
ASJC Scopus subject areas
- Molecular Medicine