3H-Imipramine binding in the blood platelets of normal twins

Ramesh C. Arora; Herbert Y. Meltzer

doi:10.1016/0165-1781(90)90031-Y

³H-Imipramine binding in the blood platelets of normal twins

Ramesh C. Arora^*, Herbert Y. Meltzer

^*Corresponding author for this work

Psychiatry and Behavioral Sciences

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

³H-Imipramine binding (IB) was studied in the blood platelets of 13 pairs of monozygotic (MZ) and dizygotic (DZ) twins, and 15 pairs of unrelated normal volunteers, to determine if IB is under genetic control. The intrapair variance of B_max (the maximum number of ³H-IB sites) was significantly smaller in MZ twins and unrelated control pairs than in DZ twins. The intraclass correlations (ICC) of B_max were significant for all the pairs with no difference between these correlations. The ICC of the K_d (inversely related to the affinity for ³H-imipramine) of IB was significant only for the normal control pairs and the DZ twins. These results suggest that the kinetic constants of IB in blood platelets are not under genetic control and that interassay variance significantly affects the absolute values for K_d and B_max of ³H-IB. The environmental and assay factors that influence ³H-IB may account for the numerous discrepancies in platelet ³H-IB between various research reports.

Original language	English (US)
Pages (from-to)	265-273
Number of pages	9
Journal	Psychiatry Research
Volume	32
Issue number	3
DOIs	https://doi.org/10.1016/0165-1781(90)90031-Y
State	Published - Jun 1990

Keywords

Imipramine binding
K and B
monozygotic and dizygotic twins
platelet

ASJC Scopus subject areas

Psychiatry and Mental health
Biological Psychiatry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/0165-1781(90)90031-Y

Cite this

@article{727a75a063dd4e69b98dd1c0ea32cd19,

title = "3H-Imipramine binding in the blood platelets of normal twins",

abstract = "3H-Imipramine binding (IB) was studied in the blood platelets of 13 pairs of monozygotic (MZ) and dizygotic (DZ) twins, and 15 pairs of unrelated normal volunteers, to determine if IB is under genetic control. The intrapair variance of Bmax (the maximum number of 3H-IB sites) was significantly smaller in MZ twins and unrelated control pairs than in DZ twins. The intraclass correlations (ICC) of Bmax were significant for all the pairs with no difference between these correlations. The ICC of the Kd (inversely related to the affinity for 3H-imipramine) of IB was significant only for the normal control pairs and the DZ twins. These results suggest that the kinetic constants of IB in blood platelets are not under genetic control and that interassay variance significantly affects the absolute values for Kd and Bmax of 3H-IB. The environmental and assay factors that influence 3H-IB may account for the numerous discrepancies in platelet 3H-IB between various research reports.",

keywords = "Imipramine binding, K and B, monozygotic and dizygotic twins, platelet",

author = "Arora, {Ramesh C.} and Meltzer, {Herbert Y.}",

note = "Funding Information: Acknowledgments. The research reported was supported, in part, by USPHS MH-30,059; MH-41684, and by the Cleveland Foundation. H.Y.M. is recipient of USPHS Research Career Scientist Award 47808. We are grateful to Ms. L. Kregel and Mrs. V. Wunnicke for laboratory assistance. Dr. J. Locascio and Mr. Jeff Sharpe for data analysis, and Ms. L. Mason for typing.",

year = "1990",

month = jun,

doi = "10.1016/0165-1781(90)90031-Y",

language = "English (US)",

volume = "32",

pages = "265--273",

journal = "Psychiatry Research",

issn = "0165-1781",

publisher = "Elsevier Ireland Ltd",

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TY - JOUR

T1 - 3H-Imipramine binding in the blood platelets of normal twins

AU - Arora, Ramesh C.

AU - Meltzer, Herbert Y.

N1 - Funding Information: Acknowledgments. The research reported was supported, in part, by USPHS MH-30,059; MH-41684, and by the Cleveland Foundation. H.Y.M. is recipient of USPHS Research Career Scientist Award 47808. We are grateful to Ms. L. Kregel and Mrs. V. Wunnicke for laboratory assistance. Dr. J. Locascio and Mr. Jeff Sharpe for data analysis, and Ms. L. Mason for typing.

PY - 1990/6

Y1 - 1990/6

N2 - 3H-Imipramine binding (IB) was studied in the blood platelets of 13 pairs of monozygotic (MZ) and dizygotic (DZ) twins, and 15 pairs of unrelated normal volunteers, to determine if IB is under genetic control. The intrapair variance of Bmax (the maximum number of 3H-IB sites) was significantly smaller in MZ twins and unrelated control pairs than in DZ twins. The intraclass correlations (ICC) of Bmax were significant for all the pairs with no difference between these correlations. The ICC of the Kd (inversely related to the affinity for 3H-imipramine) of IB was significant only for the normal control pairs and the DZ twins. These results suggest that the kinetic constants of IB in blood platelets are not under genetic control and that interassay variance significantly affects the absolute values for Kd and Bmax of 3H-IB. The environmental and assay factors that influence 3H-IB may account for the numerous discrepancies in platelet 3H-IB between various research reports.

AB - 3H-Imipramine binding (IB) was studied in the blood platelets of 13 pairs of monozygotic (MZ) and dizygotic (DZ) twins, and 15 pairs of unrelated normal volunteers, to determine if IB is under genetic control. The intrapair variance of Bmax (the maximum number of 3H-IB sites) was significantly smaller in MZ twins and unrelated control pairs than in DZ twins. The intraclass correlations (ICC) of Bmax were significant for all the pairs with no difference between these correlations. The ICC of the Kd (inversely related to the affinity for 3H-imipramine) of IB was significant only for the normal control pairs and the DZ twins. These results suggest that the kinetic constants of IB in blood platelets are not under genetic control and that interassay variance significantly affects the absolute values for Kd and Bmax of 3H-IB. The environmental and assay factors that influence 3H-IB may account for the numerous discrepancies in platelet 3H-IB between various research reports.

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